1yze: Difference between revisions

Revision as of 16:59, 3 May 2008

Crystal structure of the N-terminal domain of USP7/HAUSP.

OverviewOverview

USP7/HAUSP is a key regulator of p53 and Mdm2 and is targeted by the Epstein-Barr nuclear antigen 1 (EBNA1) protein of Epstein-Barr virus (EBV). We have determined the crystal structure of the p53 binding domain of USP7 alone and bound to an EBNA1 peptide. This domain is an eight-stranded beta sandwich similar to the TRAF-C domains of TNF-receptor associated factors, although the mode of peptide binding differs significantly from previously observed TRAF-peptide interactions in the sequence (DPGEGPS) and the conformation of the bound peptide. NMR chemical shift analyses of USP7 bound by EBNA1 and p53 indicated that p53 binds the same pocket as EBNA1 but makes less extensive contacts with USP7. Functional studies indicated that EBNA1 binding to USP7 can protect cells from apoptotic challenge by lowering p53 levels. The data provide a structural and conceptual framework for understanding how EBNA1 might contribute to the survival of Epstein-Barr virus-infected cells.

About this StructureAbout this Structure

1YZE is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the p53 binding domain of HAUSP/USP7 bound to Epstein-Barr nuclear antigen 1 implications for EBV-mediated immortalization., Saridakis V, Sheng Y, Sarkari F, Holowaty MN, Shire K, Nguyen T, Zhang RG, Liao J, Lee W, Edwards AM, Arrowsmith CH, Frappier L, Mol Cell. 2005 Apr 1;18(1):25-36. PMID:15808506 Page seeded by OCA on Sat May 3 16:59:56 2008

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OCA

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 [[Image:1yze.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1yze", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= USP7, HAUSP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_1yze|  PDB=1yze  |  SCENE=  }}
-|RELATEDENTRY=[[1yy6|1YY6]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yze OCA], [http://www.ebi.ac.uk/pdbsum/1yze PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yze RCSB]</span>
-}}
 '''Crystal structure of the N-terminal domain of USP7/HAUSP.'''
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 [[Category: Shire, K.]]
 [[Category: Zhang, R G.]]
-[[Category: apo form]]
+[[Category: Apo form]]
-[[Category: deubiquitinating enzyme]]
+[[Category: Deubiquitinating enzyme]]
-[[Category: traf domain]]
+[[Category: Traf domain]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:59:56 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:26:49 2008''