1ys4: Difference between revisions

Revision as of 16:42, 3 May 2008

Structure of Aspartate-Semialdehyde Dehydrogenase from Methanococcus jannaschii

OverviewOverview

The structure of aspartate-beta-semialdehyde dehydrogenase (ASADH) from Methanococcus jannaschii has been determined to 2.3 angstroms resolution using multiwavelength anomalous diffraction (MAD) phasing of a selenomethionine-substituted derivative to define a new branch in the family of ASADHs. This new structure has a similar overall fold and domain organization despite less than 10% conserved sequence identity with the bacterial enzymes. However, the entire repertoire of functionally important active site amino acid residues is conserved, suggesting an identical catalytic mechanism but with lower catalytic efficiency. A new coenzyme-binding conformation and dual NAD/NADP coenzyme specificity further distinguish this archaeal branch from the bacterial ASADHs. Several structural differences are proposed to account for the dramatically enhanced thermostability of this archaeal enzyme. Finally, the intersubunit communication channel connecting the active sites in the bacterial enzyme dimer has been disrupted in the archaeal ASADHs by amino acid changes that likely prevent the alternating sites reactivity previously proposed for the bacterial ASADHs.

About this StructureAbout this Structure

1YS4 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

ReferenceReference

A new branch in the family: structure of aspartate-beta-semialdehyde dehydrogenase from Methanococcus jannaschii., Faehnle CR, Ohren JF, Viola RE, J Mol Biol. 2005 Nov 11;353(5):1055-68. Epub 2005 Sep 29. PMID:16225889 Page seeded by OCA on Sat May 3 16:42:52 2008

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OCA

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 [[Image:1ys4.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1ys4", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate-semialdehyde_dehydrogenase Aspartate-semialdehyde dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.11 1.2.1.11] </span>
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-|GENE= asd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
+-->
-|DOMAIN=
+{{STRUCTURE_1ys4|  PDB=1ys4  |  SCENE=  }}
-|RELATEDENTRY=[[1brm|1BRM]], [[1nwc|1NWC]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ys4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ys4 OCA], [http://www.ebi.ac.uk/pdbsum/1ys4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ys4 RCSB]</span>
-}}
 '''Structure of Aspartate-Semialdehyde Dehydrogenase from Methanococcus jannaschii'''
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 [[Category: Ohren, J F.]]
 [[Category: Viola, R E.]]
-[[Category: asadh]]
+[[Category: Asadh]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:42:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:20:12 2008''