1ymo: Difference between revisions

Revision as of 16:31, 3 May 2008

Solution structure of the P2b-P3 pseudoknot from human telomerase RNA

OverviewOverview

Human telomerase contains a 451 nt RNA (hTR) and several proteins, including a specialized reverse transcriptase (hTERT). The 5' half of hTR comprises the pseudoknot (core) domain, which includes the RNA template for telomere synthesis and a highly conserved pseudoknot that is required for telomerase activity. The solution structure of this essential pseudoknot, presented here, reveals an extended triple helix surrounding the helical junction. The network of tertiary interactions explains the phylogenetic sequence conservation and existing human and mouse TR functional studies as well as mutations linked to disease. Thermodynamic stability, dimerization potential, and telomerase activity of mutant RNAs that alter the tertiary contacts were investigated. Telomerase activity is strongly correlated with tertiary structure stability, whereas there is no correlation with dimerization potential of the pseudoknot. These studies reveal that a conserved pseudoknot tertiary structure is required for telomerase activity.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Structure of the human telomerase RNA pseudoknot reveals conserved tertiary interactions essential for function., Theimer CA, Blois CA, Feigon J, Mol Cell. 2005 Mar 4;17(5):671-82. PMID:15749017 Page seeded by OCA on Sat May 3 16:31:00 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ymo.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1ymo", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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-|GENE=
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ymo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ymo OCA], [http://www.ebi.ac.uk/pdbsum/1ymo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ymo RCSB]</span>
-}}
 '''Solution structure of the P2b-P3 pseudoknot from human telomerase RNA'''
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 ==About this Structure==
-YMO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMO OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMO OCA].
 ==Reference==
 Structure of the human telomerase RNA pseudoknot reveals conserved tertiary interactions essential for function., Theimer CA, Blois CA, Feigon J, Mol Cell. 2005 Mar 4;17(5):671-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15749017 15749017]
-[[Category: Protein complex]]
 [[Category: Blois, C A.]]
 [[Category: Feigon, J.]]
 [[Category: Theimer, C A.]]
-[[Category: hoogsteen,triplex]]
+[[Category: Hoogsteen,triplex]]
-[[Category: non-canonical]]
+[[Category: Non-canonical]]
-[[Category: pseudoknot]]
+[[Category: Pseudoknot]]
-[[Category: tertiary structure]]
+[[Category: Tertiary structure]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:31:00 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:13:21 2008''