1ye5: Difference between revisions

Revision as of 16:12, 3 May 2008

Crystal structure of hypothetical protein of unknown function from pyrococcus horikoshii OT3

OverviewOverview

The Pyrococcus horikoshii OT3 protein PH0500 is highly conserved within the Pyrococcus genus of hyperthermophilic archaea and shows low amino-acid sequence similarity with a family of PIN-domain proteins. The protein has been expressed, purified and crystallized in two crystal forms: PH0500-I and PH0500-II. The structure was determined at 2.0 A by the multiple anomalous dispersion method using a selenomethionyl derivative of crystal form PH0500-I (PH0500-I-Se). The structure of PH0500-I has been refined at 1.75 A resolution to an R factor of 20.9% and the structure of PH0500-II has been refined at 2.0 A resolution to an R factor of 23.4%. In both crystal forms as well as in solution the molecule appears to be a dimer. Searches of the databases for protein-fold similarities confirmed that the PH0500 protein is a PIN-domain protein with possible exonuclease activity and involvement in DNA or RNA editing.

About this StructureAbout this Structure

1YE5 is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

ReferenceReference

Structure of PIN-domain protein PH0500 from Pyrococcus horikoshii., Jeyakanthan J, Inagaki E, Kuroishi C, Tahirov TH, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt, 5):463-8. Epub 2005 Apr 26. PMID:16511069 Page seeded by OCA on Sat May 3 16:12:46 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ye5.gif|left|200px]]
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 '''Crystal structure of hypothetical protein of unknown function from pyrococcus horikoshii OT3'''
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 [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
 [[Category: Tahirov, T H.]]
-[[Category: nucleotide binding protein]]
+[[Category: Nucleotide binding protein]]
-[[Category: pin domain]]
+[[Category: Pin domain]]
-[[Category: riken structural genomics/proteomics initiative]]
+[[Category: Riken structural genomics/proteomics initiative]]
-[[Category: rossmann fold]]
+[[Category: Rossmann fold]]
-[[Category: rsgi]]
+[[Category: Rsgi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: trna synthetase]]
+[[Category: Trna synthetase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:12:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:04:35 2008''