1y1v: Difference between revisions

Revision as of 15:47, 3 May 2008

Refined RNA Polymerase II-TFIIS complex

OverviewOverview

The crystal structure of the complete 12 subunit RNA polymerase (pol) II bound to a transcription bubble and product RNA reveals incoming template and nontemplate DNA, a seven base pair DNA/RNA hybrid, and three nucleotides each of separating DNA and RNA. The complex adopts the posttranslocation state and accommodates a cocrystallized nucleoside triphosphate (NTP) substrate. The NTP binds in the active site pore at a position to interact with a DNA template base. Residues surrounding the NTP are conserved in all cellular RNA polymerases, suggesting a universal mechanism of NTP selection and incorporation. DNA-DNA and DNA-RNA strand separation may be explained by pol II-induced duplex distortions. Four protein loops partition the active center cleft, contribute to embedding the hybrid, prevent strand reassociation, and create an RNA exit tunnel. Binding of the elongation factor TFIIS realigns RNA in the active center, possibly converting the elongation complex to an alternative state less prone to stalling.

About this StructureAbout this Structure

1Y1V is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS., Kettenberger H, Armache KJ, Cramer P, Mol Cell. 2004 Dec 22;16(6):955-65. PMID:15610738 Page seeded by OCA on Sat May 3 15:47:07 2008

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OCA

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 [[Image:1y1v.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1y1v", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
+or leave the SCENE parameter empty for the default display.
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y1v OCA], [http://www.ebi.ac.uk/pdbsum/1y1v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y1v RCSB]</span>
-}}
 '''Refined RNA Polymerase II-TFIIS complex'''
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 [[Category: Cramer, P.]]
 [[Category: Kettenberger, H.]]
-[[Category: elongation]]
+[[Category: Elongation]]
-[[Category: rna polymerase ii]]
+[[Category: Rna polymerase ii]]
-[[Category: tfii]]
+[[Category: Tfii]]
-[[Category: transcription]]
+[[Category: Transcription]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 15:47:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:57:42 2008''