1xu1: Difference between revisions

Revision as of 15:30, 3 May 2008

The crystal structure of APRIL bound to TACI

OverviewOverview

TACI is a member of the tumor necrosis factor receptor superfamily and serves as a key regulator of B cell function. TACI binds two ligands, APRIL and BAFF, with high affinity and contains two cysteine-rich domains (CRDs) in its extracellular region; in contrast, BCMA and BR3, the other known high affinity receptors for APRIL and BAFF, respectively, contain only a single or partial CRD. However, another form of TACI exists wherein the N-terminal CRD is removed by alternative splicing. We find that this shorter form is capable of ligand-induced cell signaling and that the second CRD alone (TACI_d2) contains full affinity for both ligands. Furthermore, we report the solution structure and alanine-scanning mutagenesis of TACI_d2 along with co-crystal structures of APRIL.TACI_d2 and APRIL.BCMA complexes that together reveal the mechanism by which TACI engages high affinity ligand binding through a single CRD, and we highlight sources of ligand-receptor specificity within the APRIL/BAFF system.

About this StructureAbout this Structure

1XU1 is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structures of APRIL-receptor complexes: like BCMA, TACI employs only a single cysteine-rich domain for high affinity ligand binding., Hymowitz SG, Patel DR, Wallweber HJ, Runyon S, Yan M, Yin J, Shriver SK, Gordon NC, Pan B, Skelton NJ, Kelley RF, Starovasnik MA, J Biol Chem. 2005 Feb 25;280(8):7218-27. Epub 2004 Nov 12. PMID:15542592 Page seeded by OCA on Sat May 3 15:30:32 2008

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OCA

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 [[Image:1xu1.gif|left|200px]]
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-|GENE= Tnfsf13, APRIL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), TNFRSF13B, TACI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-}}
 '''The crystal structure of APRIL bound to TACI'''
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 [[Category: Yan, M.]]
 [[Category: Yin, J.]]
-[[Category: crd]]
+[[Category: Crd]]
-[[Category: cysteine-rich]]
+[[Category: Cysteine-rich]]
-[[Category: cytokine]]
+[[Category: Cytokine]]
-[[Category: jelly-roll]]
+[[Category: Jelly-roll]]
-[[Category: receptor]]
+[[Category: Receptor]]
-[[Category: tnfsf]]
+[[Category: Tnfsf]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 15:30:32 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:54:36 2008''