1xrb: Difference between revisions

Revision as of 15:24, 3 May 2008

S-ADENOSYLMETHIONINE SYNTHETASE (MAT, ATP: L-METHIONINE S-ADENOSYLTRANSFERASE, E.C.2.5.1.6) IN WHICH MET RESIDUES ARE REPLACED WITH SELENOMETHIONINE RESIDUES (MSE)

OverviewOverview

The structure of S-adenosylmethionine synthetase (MAT, ATP:L-methionine S-adenosyltransferase, EC 2.5.1.6.) from Escherichia coli has been determined at 3.0 A resolution by multiple isomorphous replacement using a uranium derivative and the selenomethionine form of the enzyme (SeMAT). The SeMAT data (9 selenomethionine residues out of 383 amino acid residues) have been found to have a sufficient phasing power to determine the structure of the 42,000 molecular weight protein by combining them with the other heavy atom derivative data (multiple isomorphous replacement). The enzyme consists of four identical subunits; two subunits form a spherical tight dimer, and pairs of these dimers form a peanut-shaped tetrameric enzyme. Each pair dimer has two active sites which are located between the subunits. Each subunit consists of three domains that are related to each other by pseudo-3-fold symmetry. The essential divalent (Mg2+/Co2+) and monovalent (K+) metal ions and one of the product, Pi ions, were found in the active site from three separate structures.

About this StructureAbout this Structure

1XRB is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of S-adenosylmethionine synthetase., Takusagawa F, Kamitori S, Misaki S, Markham GD, J Biol Chem. 1996 Jan 5;271(1):136-47. PMID:8550549 Page seeded by OCA on Sat May 3 15:24:29 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1xrb.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1xrb |SIZE=350|CAPTION= <scene name='initialview01'>1xrb</scene>, resolution 3.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1xrb", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_adenosyltransferase Methionine adenosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.6 2.5.1.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1xrb|  PDB=1xrb  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xrb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xrb OCA], [http://www.ebi.ac.uk/pdbsum/1xrb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xrb RCSB]</span>
-}}
 '''S-ADENOSYLMETHIONINE SYNTHETASE (MAT, ATP: L-METHIONINE S-ADENOSYLTRANSFERASE, E.C.2.5.1.6) IN WHICH MET RESIDUES ARE REPLACED WITH SELENOMETHIONINE RESIDUES (MSE)'''
@@ Line 30: / Line 27: @@
 [[Category: Misaki, S.]]
 [[Category: Takusagawa, F.]]
-[[Category: methyltransferase]]
+[[Category: Methyltransferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 15:24:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:53:34 2008''