1xqw: Difference between revisions

Revision as of 15:23, 3 May 2008

Crystal structure of F1-mutant S105A complex with PHE-LEU

OverviewOverview

The tricorn-interacting factor F1 of the archaeon Thermoplasma acidophilum cleaves small hydrophobic peptide products of the proteasome and tricorn protease. F1 mutants of the active site residues that are involved in substrate recognition and catalysis displayed distinct activity patterns toward fluorogenic test substrates. Crystal structures of the mutant proteins complexed with peptides Phe-Leu, Pro-Pro, or Pro-Leu-Gly-Gly showed interaction of glutamates 213 and 245 with the N termini of the peptides and defined the S1 and S1' sites and the role of the catalytic residues. Evidence was found for processive peptide cleavage in the N-to-C direction, whereby the P1' product is translocated into the S1 site. A functional interaction of F1 with the tricorn protease was observed with the inactive F1 mutant G37A. Moreover, small angle x-ray scattering measurements for tricorn and inhibited F1 have been interpreted as formation of transient and substrate-induced complexes.

About this StructureAbout this Structure

1XQW is a Single protein structure of sequence from Thermoplasma acidophilum. Full crystallographic information is available from OCA.

ReferenceReference

X-ray snapshots of peptide processing in mutants of tricorn-interacting factor F1 from Thermoplasma acidophilum., Goettig P, Brandstetter H, Groll M, Gohring W, Konarev PV, Svergun DI, Huber R, Kim JS, J Biol Chem. 2005 Sep 30;280(39):33387-96. Epub 2005 Jul 1. PMID:15994304 Page seeded by OCA on Sat May 3 15:23:17 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1xqw.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1xqw |SIZE=350|CAPTION= <scene name='initialview01'>1xqw</scene>, resolution 2.00&Aring;
+The line below this paragraph, containing "STRUCTURE_1xqw", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Prolyl_aminopeptidase Prolyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.5 3.4.11.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= TA0830 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2303 Thermoplasma acidophilum])
+-->
-|DOMAIN=
+{{STRUCTURE_1xqw|  PDB=1xqw  |  SCENE=  }}
-|RELATEDENTRY=[[1mtz|1MTZ]], [[1mu0|1MU0]], [[1mt3|1MT3]], [[1xqv|1XQV]], [[1xqx|1XQX]], [[1xqy|1XQY]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xqw OCA], [http://www.ebi.ac.uk/pdbsum/1xqw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xqw RCSB]</span>
-}}
 '''Crystal structure of F1-mutant S105A complex with PHE-LEU'''
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 [[Category: Konarev, P V.]]
 [[Category: Svergun, D I.]]
-[[Category: alpha-beta hydrolase]]
+[[Category: Alpha-beta hydrolase]]
-[[Category: caged active site]]
+[[Category: Caged active site]]
-[[Category: catalytic triad]]
+[[Category: Catalytic triad]]
-[[Category: nucleophile]]
+[[Category: Nucleophile]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 15:23:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:53:23 2008''