1xmn: Difference between revisions

Revision as of 15:13, 3 May 2008

Crystal structure of thrombin bound to heparin

OverviewOverview

Thrombin is the final protease in the blood coagulation cascade and serves both pro- and anticoagulant functions through the cleavage of several targets. The ability of thrombin to specifically recognize a wide range of substrates derives from interactions that occur outside of the active site of thrombin. Thrombin possesses two anion binding exosites, which mediate many of its interactions with cofactors and substrates, and although many structures of thrombin have been solved, few such interactions have been described in molecular detail. Glycosaminoglycan binding to exosite II of thrombin plays a major role in switching off the procoagulant functions of thrombin by mediating its irreversible inhibition by circulating serpins and by its binding to the endothelial cell surface receptor thrombomodulin. Here we report the 1.85-A structure of human alpha-thrombin bound to a heparin fragment of eight monosaccharide units in length. The asymmetric unit is composed of two thrombin dimers, each sharing a single heparin octasaccharide chain. The observed interactions are fully consistent with previous mutagenesis studies and illustrate on a molecular level the cofactor interaction that is critical for the restriction of clotting to the site of blood vessel injury.

About this StructureAbout this Structure

1XMN is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of thrombin bound to heparin., Carter WJ, Cama E, Huntington JA, J Biol Chem. 2005 Jan 28;280(4):2745-9. Epub 2004 Nov 17. PMID:15548541 Page seeded by OCA on Sat May 3 15:13:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1xmn.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1xmn |SIZE=350|CAPTION= <scene name='initialview01'>1xmn</scene>, resolution 1.85&Aring;
+The line below this paragraph, containing "STRUCTURE_1xmn", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=DPN:D-PHENYLALANINE'>DPN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IDS:O2-SULFO-GLUCURONIC+ACID'>IDS</scene>, <scene name='pdbligand=MAI:DEOXO-METHYLARGININE'>MAI</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SGN:N,O6-DISULFO-GLUCOSAMINE'>SGN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thrombin Thrombin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.5 3.4.21.5] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1xmn|  PDB=1xmn  |  SCENE=  }}
-|RELATEDENTRY=[[1ppb|1PPB]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xmn OCA], [http://www.ebi.ac.uk/pdbsum/1xmn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xmn RCSB]</span>
-}}
 '''Crystal structure of thrombin bound to heparin'''
@@ Line 29: / Line 26: @@
 [[Category: Carter, W J.]]
 [[Category: Huntington, J A.]]
-[[Category: blood clotting]]
+[[Category: Blood clotting]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 15:13:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:51:43 2008''