1xme: Difference between revisions

Revision as of 15:13, 3 May 2008

Structure of Recombinant Cytochrome ba3 Oxidase from Thermus thermophilus

OverviewOverview

Cytochrome ba(3) oxidase is an integral membrane protein identified in the thermophilic bacterium Thermus thermophilus. The enzyme has now been expressed recombinantly and purified with a histidine tag. As such, it crystallizes under similar conditions and in the same space group (P4(3)2(1)2) as the native protein. A novel cryoprotection scheme is described here to obtain high-resolution diffraction from these crystals, which involves soaking in a mixture of glycerol and ethylene glycol under a layer of oil. The unit-cell parameters for these crystals are larger than the native protein, apparently deriving from increased ordering of the N-terminus and an internal loop (residues 495-500) in subunit I. Hence, compared with native cytochrome ba(3) oxidase, the recombinant His-tagged protein is accommodated in an expanded but equally well ordered lattice via an alternate set of specific intermolecular contacts. The structure was refined against data to 2.3 angstroms resolution to an R factor of 21.7% and an R(free) of 23.7%.

About this StructureAbout this Structure

1XME is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

A novel cryoprotection scheme for enhancing the diffraction of crystals of recombinant cytochrome ba3 oxidase from Thermus thermophilus., Hunsicker-Wang LM, Pacoma RL, Chen Y, Fee JA, Stout CD, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):340-3. Epub 2005, Feb 24. PMID:15735345 Page seeded by OCA on Sat May 3 15:13:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1xme.gif|left|200px]]
- {{Structure
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-|GENE= cbaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus]), cbaB, ctaC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus]), cbaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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 '''Structure of Recombinant Cytochrome ba3 Oxidase from Thermus thermophilus'''
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 [[Category: Pacoma, R L.]]
 [[Category: Stout, C D.]]
-[[Category: cytochrome oxidase]]
+[[Category: Cytochrome oxidase]]
-[[Category: heme]]
+[[Category: Heme]]
-[[Category: heme-a]]
+[[Category: Heme-a]]
-[[Category: integral membrane protein]]
+[[Category: Integral membrane protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 15:13:11 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:51:39 2008''