1x75: Difference between revisions

Revision as of 14:39, 3 May 2008

CcdB:GyrA14 complex

OverviewOverview

Gyrase is an ubiquitous bacterial enzyme that is responsible for disentangling DNA during DNA replication and transcription. It is the target of the toxin CcdB, a paradigm for plasmid addiction systems and related bacterial toxin-antitoxin systems. The crystal structure of CcdB and the dimerization domain of the A subunit of gyrase (GyrA14) dictates an open conformation for the catalytic domain of gyrase when CcdB is bound. The action of CcdB is one of a wedge that stabilizes a dead-end covalent gyrase:DNA adduct. Although CcdB and GyrA14 form a globally symmetric complex where the two 2-fold axes of both dimers align, the complex is asymmetric in its details. At the centre of the interaction site, the Trp99 pair of CcdB stacks with the Arg462 pair of GyrA14, explaining why the Arg462Cys mutation in the A subunit of gyrase confers resistance to CcdB. Overexpression of GyrA14 protects Escherichia coli cells against CcdB, mimicking the action of the antidote CcdA.

About this StructureAbout this Structure

1X75 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis of gyrase poisoning by the addiction toxin CcdB., Dao-Thi MH, Van Melderen L, De Genst E, Afif H, Buts L, Wyns L, Loris R, J Mol Biol. 2005 May 20;348(5):1091-102. Epub 2005 Apr 7. PMID:15854646 Page seeded by OCA on Sat May 3 14:39:04 2008

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OCA

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 [[Image:1x75.gif|left|200px]]
- {{Structure
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-}}
 '''CcdB:GyrA14 complex'''
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 ==Reference==
 Molecular basis of gyrase poisoning by the addiction toxin CcdB., Dao-Thi MH, Van Melderen L, De Genst E, Afif H, Buts L, Wyns L, Loris R, J Mol Biol. 2005 May 20;348(5):1091-102. Epub 2005 Apr 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15854646 15854646]
-[[Category: DNA topoisomerase (ATP-hydrolyzing)]]
 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
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 [[Category: Melderen, L Van.]]
 [[Category: Wyns, L.]]
-[[Category: bacterial cell death]]
+[[Category: Bacterial cell death]]
-[[Category: ccdb]]
+[[Category: Ccdb]]
-[[Category: gyrase]]
+[[Category: Gyrase]]
-[[Category: plasmid addiction]]
+[[Category: Plasmid addiction]]
-[[Category: ta system]]
+[[Category: Ta system]]
-[[Category: topoisomerase]]
+[[Category: Topoisomerase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 14:39:04 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:45:37 2008''