1wtb: Difference between revisions

Revision as of 14:06, 3 May 2008

Complex structure of the C-terminal RNA-binding domain of hnRNP D (AUF1) with telomere DNA

OverviewOverview

Heterogeneous nuclear ribonucleoprotein D, also known as AUF1, has two DNA/RNA-binding domains, each of which can specifically bind to single-stranded d(TTAGGG)n, the human telomeric repeat. Here, the structure of the C-terminal-binding domain (BD2) complexed with single-stranded d(TTAGGG) determined by NMR is presented. The structure has revealed that each residue of the d(TAG) segment is recognized by BD2 in a base-specific manner. The interactions deduced from the structure have been confirmed by gel retardation experiments with mutant BD2 and DNA. It is known that single-stranded DNA with the telomeric repeat tends to form a quadruplex and that the quadruplex has an inhibitory effect on telomere elongation by telomerase. This time it is revealed that BD2 unfolds the quadruplex of such DNA upon binding. Moreover, the effect of BD2 on the elongation by telomerase was examined in vitro. These results suggest the possible involvement of heterogeneous nuclear ribonucleoprotein D in maintenance of the telomere 3'-overhang either through protection of a single-stranded DNA or destabilization of the potentially deleterious quadruplex structure for the elongation by telomerase.

About this StructureAbout this Structure

1WTB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of hnRNP D complexed with single-stranded telomere DNA and unfolding of the quadruplex by heterogeneous nuclear ribonucleoprotein D., Enokizono Y, Konishi Y, Nagata K, Ouhashi K, Uesugi S, Ishikawa F, Katahira M, J Biol Chem. 2005 May 13;280(19):18862-70. Epub 2005 Feb 24. PMID:15734733 Page seeded by OCA on Sat May 3 14:06:31 2008

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OCA

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 [[Image:1wtb.gif|left|200px]]
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 '''Complex structure of the C-terminal RNA-binding domain of hnRNP D (AUF1) with telomere DNA'''
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 [[Category: Ouhashi, K.]]
 [[Category: Uesugi, S.]]
-[[Category: auf1]]
+[[Category: Auf1]]
-[[Category: complex]]
+[[Category: Complex]]
-[[Category: dna-binding domain]]
+[[Category: Dna-binding domain]]
-[[Category: hnrnp d]]
+[[Category: Hnrnp d]]
-[[Category: rna-binding domain]]
+[[Category: Rna-binding domain]]
-[[Category: rrm]]
+[[Category: Rrm]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: telomere]]
+[[Category: Telomere]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 14:06:31 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:40:41 2008''