1woe: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1woe.gif|left|200px]] | [[Image:1woe.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1woe", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1woe| PDB=1woe | SCENE= }} | |||
}} | |||
'''X-ray structure of a Z-DNA hexamer d(CGCGCG)''' | '''X-ray structure of a Z-DNA hexamer d(CGCGCG)''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WOE OCA]. | |||
==Reference== | ==Reference== | ||
The hydration structure of a Z-DNA hexameric duplex determined by a neutron diffraction technique., Chatake T, Tanaka I, Umino H, Arai S, Niimura N, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1088-98. Epub 2005, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16041074 16041074] | The hydration structure of a Z-DNA hexameric duplex determined by a neutron diffraction technique., Chatake T, Tanaka I, Umino H, Arai S, Niimura N, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1088-98. Epub 2005, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16041074 16041074] | ||
[[Category: Arai, S.]] | [[Category: Arai, S.]] | ||
[[Category: Chatake, T.]] | [[Category: Chatake, T.]] | ||
| Line 29: | Line 25: | ||
[[Category: Tanaka, I.]] | [[Category: Tanaka, I.]] | ||
[[Category: Umino, H.]] | [[Category: Umino, H.]] | ||
[[Category: | [[Category: Heavy water]] | ||
[[Category: | [[Category: Spermine]] | ||
[[Category: | [[Category: Z-dna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:56:40 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 13:56, 3 May 2008
X-ray structure of a Z-DNA hexamer d(CGCGCG)
OverviewOverview
In order to reveal the hydration structure of Z-DNA, a neutron diffraction study has been carried out at 1.8 A resolution on a Z-DNA hexamer d(CGCGCG). Neutron diffraction data were collected with the BIX-3 single-crystal diffractometer at the JRR-3 reactor in the Japan Atomic Energy Research Institute (JAERI) using a large crystal (1.6 mm3) obtained from D2O solution. It has been found that almost all the guanine bases have participated in H/D exchange at the C8-H8 group, consistent with the acidic nature of this bond. 44 water molecules were found in the nuclear density maps, of which 29 showed the entire contour of all three atoms (D-O-D). The remaining 15 water molecules had a simple spherical shape, indicating that they were rotationally disordered. An interesting relationship was found between the orientational disorder of the water molecules and their locations. Almost all water molecules in the minor groove were well ordered in the crystal, while 40% of the water molecules in the major groove were rotationally disordered. The hydrogen-bonding networks in the hydration shells have two structural aspects: flexibility and regularity.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
The hydration structure of a Z-DNA hexameric duplex determined by a neutron diffraction technique., Chatake T, Tanaka I, Umino H, Arai S, Niimura N, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1088-98. Epub 2005, Jul 20. PMID:16041074 Page seeded by OCA on Sat May 3 13:56:40 2008