1wid: Difference between revisions

Revision as of 13:43, 3 May 2008

Solution Structure of the B3 DNA-Binding Domain of RAV1

OverviewOverview

The B3 DNA binding domain is shared amongst various plant-specific transcription factors, including factors involved in auxin-regulated and abscisic acid-regulated transcription. Herein, we report the NMR solution structure of the B3 domain of the Arabidopsis thaliana cold-responsive transcription factor RAV1. The structure consists of a seven-stranded open beta-barrel and two alpha-helices located at the ends of the barrel and is significantly similar to the structure of the noncatalytic DNA binding domain of the restriction enzyme EcoRII. An NMR titration experiment revealed a DNA recognition interface that enabled us to propose a structural model of the protein-DNA complex. The locations of the DNA-contacting residues are also likely to be similar to those of the EcoRII DNA binding domain.

About this StructureAbout this Structure

1WID is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

ReferenceReference

Solution structure of the B3 DNA binding domain of the Arabidopsis cold-responsive transcription factor RAV1., Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Hayami N, Terada T, Shirouzu M, Osanai T, Tanaka A, Seki M, Shinozaki K, Yokoyama S, Plant Cell. 2004 Dec;16(12):3448-59. Epub 2004 Nov 17. PMID:15548737 Page seeded by OCA on Sat May 3 13:43:07 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1wid.jpg|left|200px]]
- {{Structure
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-|GENE= RAV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])
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 '''Solution Structure of the B3 DNA-Binding Domain of RAV1'''
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 [[Category: Yamasaki, K.]]
 [[Category: Yokoyama, S.]]
-[[Category: dna-binding domain]]
+[[Category: Dna-binding domain]]
-[[Category: riken structural genomics/proteomics initiative]]
+[[Category: Riken structural genomics/proteomics initiative]]
-[[Category: rsgi]]
+[[Category: Rsgi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:43:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:36:40 2008''