1wc1: Difference between revisions

Revision as of 13:26, 3 May 2008

SOLUBLE ADENYLYL CYCLASE CYAC FROM S. PLATENSIS IN COMPLEX WITH RP-ATPALPHAS

OverviewOverview

In an evolutionarily conserved signaling pathway, 'soluble' adenylyl cyclases (sACs) synthesize the ubiquitous second messenger cyclic adenosine 3',5'-monophosphate (cAMP) in response to bicarbonate and calcium signals. Here, we present crystal structures of a cyanobacterial sAC enzyme in complex with ATP analogs, calcium and bicarbonate, which represent distinct catalytic states of the enzyme. The structures reveal that calcium occupies the first ion-binding site and directly mediates nucleotide binding. The single ion-occupied, nucleotide-bound state defines a novel, open adenylyl cyclase state. In contrast, bicarbonate increases the catalytic rate by inducing marked active site closure and recruiting a second, catalytic ion. The phosphates of the bound substrate analogs are rearranged, which would facilitate product formation and release. The mechanisms of calcium and bicarbonate sensing define a reaction pathway involving active site closure and metal recruitment that may be universal for class III cyclases.

About this StructureAbout this Structure

1WC1 is a Single protein structure of sequence from Arthrospira platensis. Full crystallographic information is available from OCA.

ReferenceReference

Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment., Steegborn C, Litvin TN, Levin LR, Buck J, Wu H, Nat Struct Mol Biol. 2005 Jan;12(1):32-7. Epub 2004 Dec 26. PMID:15619637 Page seeded by OCA on Sat May 3 13:26:46 2008

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OCA

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 [[Image:1wc1.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1wc1 |SIZE=350|CAPTION= <scene name='initialview01'>1wc1</scene>, resolution 1.93&Aring;
+The line below this paragraph, containing "STRUCTURE_1wc1", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+C'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAT:ADENOSINE-5&#39;-RP-ALPHA-THIO-TRIPHOSPHATE'>TAT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1wc1|  PDB=1wc1  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wc1 OCA], [http://www.ebi.ac.uk/pdbsum/1wc1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wc1 RCSB]</span>
-}}
 '''SOLUBLE ADENYLYL CYCLASE CYAC FROM S. PLATENSIS IN COMPLEX WITH RP-ATPALPHAS'''
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 [[Category: Steegborn, C.]]
 [[Category: Wu, H.]]
-[[Category: camp signaling]]
+[[Category: Camp signaling]]
-[[Category: cyclase]]
+[[Category: Cyclase]]
-[[Category: lyase]]
+[[Category: Lyase]]
-[[Category: soluble adenylyl cyclase]]
+[[Category: Soluble adenylyl cyclase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:26:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:34:13 2008''