1w7c: Difference between revisions

Revision as of 13:15, 3 May 2008

PPLO AT 1.23 ANGSTROMS

OverviewOverview

The structure of Pichia pastoris lysyl oxidase (PPLO) in a new crystal form has been refined at 1.23 Angstrom resolution. PPLO, a copper amine oxidase (CuAO) with a 2,4,5-trihydroxyphenylalanine quinone (TPQ) cofactor, differs from most other members of the CuAO enzyme family in having the ability to oxidize the side chain of lysine residues in a polypeptide. In the asymmetric unit of the crystals, the structure analysis has located residues 43-779 of the polypeptide chain, seven carbohydrate residues, the active-site Cu atom, an imidazole molecule bound at the active site, two buried Ca(2+) ions, five surface Mg(2+) ions, five surface Cl(-) ions and 1045 water molecules. The crystallographic residuals are R = 0.112 and R(free) = 0.146. The TPQ cofactor and several other active-site residues are poorly ordered, in contrast to the surrounding well ordered structure. A covalent cross-link is observed between two lysine residues, Lys778 and Lys66. The cross-link is likely to have been formed by the oxidation of Lys778 followed by a spontaneous reaction with Lys66. The link is modelled as dehydrolysinonorleucine.

About this StructureAbout this Structure

1W7C is a Single protein structure of sequence from Pichia pastoris. Full crystallographic information is available from OCA.

ReferenceReference

The 1.23 Angstrom structure of Pichia pastoris lysyl oxidase reveals a lysine-lysine cross-link., Duff AP, Cohen AE, Ellis PJ, Hilmer K, Langley DB, Dooley DM, Freeman HC, Guss JM, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1073-84. Epub 2006, Aug 19. PMID:16929109 Page seeded by OCA on Sat May 3 13:15:30 2008

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OCA

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 [[Image:1w7c.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1w7c |SIZE=350|CAPTION= <scene name='initialview01'>1w7c</scene>, resolution 1.23&Aring;
+The line below this paragraph, containing "STRUCTURE_1w7c", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-lysine_6-oxidase Protein-lysine 6-oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.13 1.4.3.13] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1w7c|  PDB=1w7c  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w7c OCA], [http://www.ebi.ac.uk/pdbsum/1w7c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w7c RCSB]</span>
-}}
 '''PPLO AT 1.23 ANGSTROMS'''
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 [[Category: Ellis, P J.]]
 [[Category: Guss, J M.]]
-[[Category: amne oxidase]]
+[[Category: Amne oxidase]]
-[[Category: copper]]
+[[Category: Copper]]
-[[Category: oxidase]]
+[[Category: Oxidase]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: quinoprotein]]
+[[Category: Quinoprotein]]
-[[Category: topaquinone enzyme]]
+[[Category: Topaquinone enzyme]]
-[[Category: tpq]]
+[[Category: Tpq]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 13:15:30 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:32:11 2008''