1vz3: Difference between revisions

Revision as of 12:56, 3 May 2008

PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, T597C MUTANT

OverviewOverview

Prolyl oligopeptidase contains a peptidase domain and its catalytic triad is covered by the central tunnel of a seven-bladed beta-propeller. This domain makes the enzyme an oligopeptidase by excluding large structured peptides from the active site. The apparently rigid crystal structure does not explain how the substrate can approach the catalytic groups. Two possibilities of substrate access were investigated: either blades 1 and 7 of the propeller domain move apart, or the peptidase and/or propeller domains move to create an entry site at the domain interface. Engineering disulfide bridges to the expected oscillating structures prevented such movements, which destroyed the catalytic activity and precluded substrate binding. This indicated that concerted movements of the propeller and the peptidase domains are essential for the enzyme action.

About this StructureAbout this Structure

1VZ3 is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

ReferenceReference

Concerted structural changes in the peptidase and the propeller domains of prolyl oligopeptidase are required for substrate binding., Szeltner Z, Rea D, Juhasz T, Renner V, Fulop V, Polgar L, J Mol Biol. 2004 Jul 9;340(3):627-37. PMID:15210359 Page seeded by OCA on Sat May 3 12:56:24 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1vz3.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1vz3 |SIZE=350|CAPTION= <scene name='initialview01'>1vz3</scene>, resolution 1.6&Aring;
+The line below this paragraph, containing "STRUCTURE_1vz3", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AS1:Engineered+Disulphide+Bridge'>AS1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1vz3|  PDB=1vz3  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vz3 OCA], [http://www.ebi.ac.uk/pdbsum/1vz3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vz3 RCSB]</span>
-}}
 '''PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, T597C MUTANT'''
@@ Line 28: / Line 25: @@
 [[Category: Fulop, V.]]
 [[Category: Rea, D.]]
-[[Category: alpha/ beta-hydrolase]]
+[[Category: Alpha/ beta-hydrolase]]
-[[Category: amnesia]]
+[[Category: Amnesia]]
-[[Category: beta-propeller]]
+[[Category: Beta-propeller]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: prolyl oligopeptidase]]
+[[Category: Prolyl oligopeptidase]]
-[[Category: serine protease]]
+[[Category: Serine protease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:56:24 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:28:55 2008''