1vyh: Difference between revisions

Revision as of 12:54, 3 May 2008

PAF-AH HOLOENZYME: LIS1/ALFA2

OverviewOverview

Mutations in the LIS1 gene cause lissencephaly, a human neuronal migration disorder. LIS1 binds dynein and the dynein-associated proteins Nde1 (formerly known as NudE), Ndel1 (formerly known as NUDEL), and CLIP-170, as well as the catalytic alpha dimers of brain cytosolic platelet activating factor acetylhydrolase (PAF-AH). The mechanism coupling the two diverse regulatory pathways remains unknown. We report the structure of LIS1 in complex with the alpha2/alpha2 PAF-AH homodimer. One LIS1 homodimer binds symmetrically to one alpha2/alpha2 homodimer via the highly conserved top faces of the LIS1 beta propellers. The same surface of LIS1 contains sites of mutations causing lissencephaly and overlaps with a putative dynein binding surface. Ndel1 competes with the alpha2/alpha2 homodimer for LIS1, but the interaction is complex and requires both the N- and C-terminal domains of LIS1. Our data suggest that the LIS1 molecule undergoes major conformational rearrangement when switching from a complex with the acetylhydrolase to the one with Ndel1.

About this StructureAbout this Structure

1VYH is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Coupling PAF signaling to dynein regulation: structure of LIS1 in complex with PAF-acetylhydrolase., Tarricone C, Perrina F, Monzani S, Massimiliano L, Kim MH, Derewenda ZS, Knapp S, Tsai LH, Musacchio A, Neuron. 2004 Dec 2;44(5):809-21. PMID:15572112 Page seeded by OCA on Sat May 3 12:54:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1vyh.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1vyh |SIZE=350|CAPTION= <scene name='initialview01'>1vyh</scene>, resolution 3.4&Aring;
+The line below this paragraph, containing "STRUCTURE_1vyh", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/1-alkyl-2-acetylglycerophosphocholine_esterase 1-alkyl-2-acetylglycerophosphocholine esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.47 3.1.1.47] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1vyh|  PDB=1vyh  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vyh OCA], [http://www.ebi.ac.uk/pdbsum/1vyh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vyh RCSB]</span>
-}}
 '''PAF-AH HOLOENZYME: LIS1/ALFA2'''
@@ Line 35: / Line 32: @@
 [[Category: Tarricone, C.]]
 [[Category: Tsai, L H.]]
-[[Category: acetylhydrolase]]
+[[Category: Acetylhydrolase]]
-[[Category: cell division]]
+[[Category: Cell division]]
-[[Category: cytoskeleton]]
+[[Category: Cytoskeleton]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: lissencephaly]]
+[[Category: Lissencephaly]]
-[[Category: mitosis]]
+[[Category: Mitosis]]
-[[Category: neurogenesis]]
+[[Category: Neurogenesis]]
-[[Category: platelet activacting factor]]
+[[Category: Platelet activacting factor]]
-[[Category: regulator of cytoplasmic dynein]]
+[[Category: Regulator of cytoplasmic dynein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:54:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:28:40 2008''