1vs3: Difference between revisions

Revision as of 12:51, 3 May 2008

Crystal Structure of the tRNA Pseudouridine Synthase TruA From Thermus thermophilus HB8

OverviewOverview

The pseudouridine synthase (Psi synthase) TruA catalyzes the conversion of uridine to pseudouridine at positions 38, 39 and/or 40 in the anticodon stem-loop (ASL) of tRNA. We have determined the crystal structure of TruA from Thermus thermophilus HB8 at 2.25 A resolution. TruA and the other (Psi synthases have a completely conserved active site aspartate, which suggests that the members of this enzyme family share a common catalytic mechanism. The T. thermophilus TruA structure reveals the remarkably flexible structural features in the tRNA-binding cleft, which may be responsible for the primary tRNA interaction. In addition, the charged residues occupying the intermediate positions in the cleft may lead the tRNA to the active site for catalysis. Based on the TruB-tRNA complex structure, the T. thermophilus TruA structure reveals that the tRNA probably makes the melting base pairs move into the cleft, and suggests that a conformational change of the substrate tRNA is necessary to facilitate access to the active site aspartate residue, deep within the cleft.

About this StructureAbout this Structure

1VS3 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of tRNA pseudouridine synthase TruA from Thermus thermophilus HB8., Dong X, Bessho Y, Shibata R, Nishimoto M, Shirouzu M, Kuramitsu S, Yokoyama S, RNA Biol. 2006 Jul;3(3):115-22. Epub 2006 Jul 17. PMID:17114947 Page seeded by OCA on Sat May 3 12:51:28 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1vs3.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1vs3 |SIZE=350|CAPTION= <scene name='initialview01'>1vs3</scene>, resolution 2.25&Aring;
+The line below this paragraph, containing "STRUCTURE_1vs3", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-pseudouridine_synthase_I tRNA-pseudouridine synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.12 5.4.99.12] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= truA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
+-->
-|DOMAIN=
+{{STRUCTURE_1vs3|  PDB=1vs3  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vs3 OCA], [http://www.ebi.ac.uk/pdbsum/1vs3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vs3 RCSB]</span>
-}}
 '''Crystal Structure of the tRNA Pseudouridine Synthase TruA From Thermus thermophilus HB8'''
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 [[Category: Single protein]]
 [[Category: Thermus thermophilus]]
-[[Category: tRNA-pseudouridine synthase I]]
+[[Category: TRNA-pseudouridine synthase I]]
 [[Category: Bessho, Y.]]
 [[Category: Dong, X.]]
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 [[Category: Shirouzu, M.]]
 [[Category: Yokoyama, S.]]
-[[Category: national project on protein structural and functional analyse]]
+[[Category: National project on protein structural and functional analyse]]
-[[Category: nppsfa]]
+[[Category: Nppsfa]]
-[[Category: pseudouridine synthase]]
+[[Category: Pseudouridine synthase]]
-[[Category: riken structural genomics/proteomics initiative]]
+[[Category: Riken structural genomics/proteomics initiative]]
-[[Category: rsgi]]
+[[Category: Rsgi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: trna modification]]
+[[Category: Trna modification]]
-[[Category: trua]]
+[[Category: Trua]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:51:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:27:57 2008''