1vqe: Difference between revisions

Revision as of 12:47, 3 May 2008

GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ILE 35 AND ILE 47 REPLACED BY MET 47 (V35I, I47M)

OverviewOverview

The problem of rationally engineering protein molecules can be simplified where effects of mutations on protein function are additive. Crystal structures of single and double mutants in the hydrophobic core of gene V protein indicate that structural and functional effects of core mutations are additive when the regions structurally influenced by the mutations do not substantially overlap. These regions of influence can provide a simple basis for identifying sets of mutations that will show additive effects.

About this StructureAbout this Structure

1VQE is a Single protein structure of sequence from Enterobacteria phage f1. Full crystallographic information is available from OCA.

ReferenceReference

Potential use of additivity of mutational effects in simplifying protein engineering., Skinner MM, Terwilliger TC, Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10753-7. PMID:8855252 Page seeded by OCA on Sat May 3 12:47:42 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1vqe.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1vqe |SIZE=350|CAPTION= <scene name='initialview01'>1vqe</scene>, resolution 1.8&Aring;
+The line below this paragraph, containing "STRUCTURE_1vqe", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1vqe|  PDB=1vqe  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vqe OCA], [http://www.ebi.ac.uk/pdbsum/1vqe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vqe RCSB]</span>
-}}
 '''GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ILE 35 AND ILE 47 REPLACED BY MET 47 (V35I, I47M)'''
@@ Line 27: / Line 24: @@
 [[Category: Skinner, M M.]]
 [[Category: Terwilliger, T C.]]
-[[Category: dna-binding protein]]
+[[Category: Dna-binding protein]]
-[[Category: gene v]]
+[[Category: Gene v]]
-[[Category: mutant]]
+[[Category: Mutant]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:47:42 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:27:18 2008''