1vgr: Difference between revisions

Revision as of 12:31, 3 May 2008

Formyl-CoA transferase mutant Asp169 to Glu

OverviewOverview

Oxalobacter formigenes is an obligate anaerobe that colonizes the human gastrointestinal tract and employs oxalate breakdown to generate ATP in a novel process involving the interplay of two coupled enzymes and a membrane-bound oxalate:formate antiporter. Formyl-CoA transferase is a critical enzyme in oxalate-dependent ATP synthesis and is the first Class III CoA-transferase for which a high resolution, three-dimensional structure has been determined (Ricagno, S., Jonsson, S., Richards, N., and Lindqvist, Y. (2003) EMBO J. 22, 3210-3219). We now report the first detailed kinetic characterizations of recombinant, wild type formyl-CoA transferase and a number of site-specific mutants, which suggest that catalysis proceeds via a series of anhydride intermediates. Further evidence for this mechanistic proposal is provided by the x-ray crystallographic observation of an acylenzyme intermediate that is formed when formyl-CoA transferase is incubated with oxalyl-CoA. The catalytic mechanism of formyl-CoA transferase is therefore established and is almost certainly employed by all other members of the Class III CoA-transferase family.

About this StructureAbout this Structure

1VGR is a Single protein structure of sequence from Oxalobacter formigenes. Full crystallographic information is available from OCA.

ReferenceReference

Kinetic and mechanistic characterization of the formyl-CoA transferase from Oxalobacter formigenes., Jonsson S, Ricagno S, Lindqvist Y, Richards NG, J Biol Chem. 2004 Aug 20;279(34):36003-12. Epub 2004 Jun 21. PMID:15213226 Page seeded by OCA on Sat May 3 12:31:26 2008

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OCA

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 [[Image:1vgr.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1vgr |SIZE=350|CAPTION= <scene name='initialview01'>1vgr</scene>, resolution 2.10&Aring;
+The line below this paragraph, containing "STRUCTURE_1vgr", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=COA:COENZYME+A'>COA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Formyl-CoA_transferase Formyl-CoA transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.16 2.8.3.16] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= FRC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=847 Oxalobacter formigenes])
+-->
-|DOMAIN=
+{{STRUCTURE_1vgr|  PDB=1vgr  |  SCENE=  }}
-|RELATEDENTRY=[[1p5h|1p5h]], [[1p5r|1p5r]], [[1vgq|1vgq]], [[1t3z|1t3z]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vgr OCA], [http://www.ebi.ac.uk/pdbsum/1vgr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vgr RCSB]</span>
-}}
 '''Formyl-CoA transferase mutant Asp169 to Glu'''
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 [[Category: Ricagno, S.]]
 [[Category: Richards, N G.]]
-[[Category: caib-baif family]]
+[[Category: Caib-baif family]]
-[[Category: coa complex]]
+[[Category: Coa complex]]
-[[Category: coa transferase]]
+[[Category: Coa transferase]]
-[[Category: intertwined]]
+[[Category: Intertwined]]
-[[Category: knotted fold]]
+[[Category: Knotted fold]]
-[[Category: oxalate]]
+[[Category: Oxalate]]
-[[Category: oxalate degradation]]
+[[Category: Oxalate degradation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:31:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:24:10 2008''