1uzr: Difference between revisions

Revision as of 11:54, 3 May 2008

CRYSTAL STRUCTURE OF THE CLASS IB RIBONUCLEOTIDE REDUCTASE R2F-2 SUBUNIT FROM MYCOBACTERIUM TUBERCULOSIS

OverviewOverview

Two nrdF genes of Mycobacterium tuberculosis code for different R2 subunits of the class Ib ribonucleotide reductase (RNR). The proteins are denoted R2F-1 and R2F-2 having 71% sequence identity. The R2F-2 subunit forms the biologically active RNR complex with the catalytic R1E-subunit. We present the structure of the reduced R2F-2 subunit to 2.2 A resolution. Comparison of the R2F-2 structure with a model of R2F-1 suggests that the important differences are located at the C-terminus. We found that within class Ib, the E-helix close to the iron diiron centre has two preferred conformations, which cannot be explained by the redox-state of the diiron centre. In the R2F-2 structure, we also could see a mobility of alphaE in between the two conformations.

About this StructureAbout this Structure

1UZR is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the biologically active form of class Ib ribonucleotide reductase small subunit from Mycobacterium tuberculosis., Uppsten M, Davis J, Rubin H, Uhlin U, FEBS Lett. 2004 Jul 2;569(1-3):117-22. PMID:15225619 Page seeded by OCA on Sat May 3 11:54:53 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1uzr.jpg|left|200px]]
- {{Structure
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uzr OCA], [http://www.ebi.ac.uk/pdbsum/1uzr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uzr RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE CLASS IB RIBONUCLEOTIDE REDUCTASE R2F-2 SUBUNIT FROM MYCOBACTERIUM TUBERCULOSIS'''
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 [[Category: Uhlin, U.]]
 [[Category: Uppsten, M.]]
-[[Category: r2f-2]]
+[[Category: R2f-2]]
-[[Category: radical generation]]
+[[Category: Radical generation]]
-[[Category: ribonucleotide reductase]]
+[[Category: Ribonucleotide reductase]]
-[[Category: small subunit]]
+[[Category: Small subunit]]
-[[Category: tuberculosis]]
+[[Category: Tuberculosis]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:54:53 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:17:41 2008''