1uz8: Difference between revisions

Revision as of 11:53, 3 May 2008

ANTI-LEWIS X FAB FRAGMENT IN COMPLEX WITH LEWIS X

OverviewOverview

The Lewis X trisaccharide is pivotal in mediating specific cell-cell interactions. Monoclonal antibody 291-2G3-A, which was generated from mice infected with schistosomes, has been shown to recognize the Lewis X trisaccharide. Here we describe the structure of the Fab fragment of 291-2G3-A, with Lewis X, to 1.8 A resolution. The crystallographic analysis revealed that the antigen binding site is a rather shallow binding pocket, and residues from all six complementary determining regions of the antibody contact all sugar residues. The high specificity of the binding pocket does not result in high affinity; the K(D) determined by isothermal calorimetry is 11 microM. However, this affinity is in the same range as for other sugar-antibody complexes. The detailed understanding of the antibody-Lewis X interaction revealed by the crystal structure may be helpful in the design of better diagnostic tools for schistosomiasis and for studying Lewis X-mediated cell-cell interactions by antibody interference.

About this StructureAbout this Structure

1UZ8 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of an anti-Lewis X Fab fragment in complex with its Lewis X antigen., van Roon AM, Pannu NS, de Vrind JP, van der Marel GA, van Boom JH, Hokke CH, Deelder AM, Abrahams JP, Structure. 2004 Jul;12(7):1227-36. PMID:15242599 Page seeded by OCA on Sat May 3 11:53:35 2008

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OCA

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 [[Image:1uz8.jpg|left|200px]]
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-}}
 '''ANTI-LEWIS X FAB FRAGMENT IN COMPLEX WITH LEWIS X'''
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 [[Category: Roon, A M.M Van.]]
 [[Category: Vrind, J P.M De.]]
-[[Category: anti-carbohydrate]]
+[[Category: Anti-carbohydrate]]
-[[Category: antibody]]
+[[Category: Antibody]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:53:35 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:17:27 2008''