1uyn: Difference between revisions

Revision as of 11:52, 3 May 2008

TRANSLOCATOR DOMAIN OF AUTOTRANSPORTER NALP FROM NEISSERIA MENINGITIDIS

OverviewOverview

Autotransporters are virulence-related proteins of Gram-negative bacteria that are secreted via an outer-membrane-based C-terminal extension, the translocator domain. This domain supposedly is sufficient for the transport of the N-terminal passenger domain across the outer membrane. We present here the crystal structure of the in vitro-folded translocator domain of the autotransporter NalP from Neisseria meningitidis, which reveals a 12-stranded beta-barrel with a hydrophilic pore of 10 x 12.5 A that is filled by an N-terminal alpha-helix. The domain has pore activity in vivo and in vitro. Our data are consistent with the model of passenger-domain transport through the hydrophilic channel within the beta-barrel, and inconsistent with a model for transport through a central channel formed by an oligomer of translocator domains. However, the dimensions of the pore imply translocation of the secreted domain in an unfolded form. An alternative model, possibly covering the transport of folded domains, is that passenger-domain transport involves the Omp85 complex, the machinery required for membrane insertion of outer-membrane proteins, on which autotransporters are dependent.

About this StructureAbout this Structure

1UYN is a Single protein structure of sequence from Neisseria meningitidis. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the translocator domain of a bacterial autotransporter., Oomen CJ, van Ulsen P, van Gelder P, Feijen M, Tommassen J, Gros P, EMBO J. 2004 Mar 24;23(6):1257-66. Epub 2004 Mar 11. PMID:15014442 Page seeded by OCA on Sat May 3 11:52:17 2008

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OCA

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 [[Image:1uyn.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uyn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uyn OCA], [http://www.ebi.ac.uk/pdbsum/1uyn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uyn RCSB]</span>
-}}
 '''TRANSLOCATOR DOMAIN OF AUTOTRANSPORTER NALP FROM NEISSERIA MENINGITIDIS'''
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 [[Category: Tommassen, J.]]
 [[Category: Ulsen, P Van.]]
-[[Category: autotransporter]]
+[[Category: Autotransporter]]
-[[Category: beta-barrel]]
+[[Category: Beta-barrel]]
-[[Category: beta-domain]]
+[[Category: Beta-domain]]
-[[Category: membrane protein]]
+[[Category: Membrane protein]]
-[[Category: outer membrane]]
+[[Category: Outer membrane]]
-[[Category: translocator domain]]
+[[Category: Translocator domain]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:52:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:17:12 2008''