1ur6: Difference between revisions

Revision as of 11:34, 3 May 2008

NMR BASED STRUCTURAL MODEL OF THE UBCH5B-CNOT4 COMPLEX

OverviewOverview

The protein CNOT4 possesses an N-terminal RING finger domain that acts as an E3 ubiquitin ligase and specifically interacts with UbcH5B, a ubiquitin-conjugating enzyme. The structure of the CNOT4 RING domain has been solved and the amino acids important for the binding to UbcH5B have been mapped. Here, the residues of UbcH5B important for the binding to CNOT4 RING domain were identified by NMR chemical shift perturbation experiments, and these data were used to generate structural models of the complex with the program HADDOCK. Together with the NMR data, additional biochemical data were included in a second docking, and comparisons of the resulting model with the structure of the c-Cbl/UbcH7 complex reveal some significant differences, notably at specific residues, and give structural insights into the E2/E3 specificity.

About this StructureAbout this Structure

1UR6 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches., Dominguez C, Bonvin AM, Winkler GS, van Schaik FM, Timmers HT, Boelens R, Structure. 2004 Apr;12(4):633-44. PMID:15062086 Page seeded by OCA on Sat May 3 11:34:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1ur6.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ur6 |SIZE=350|CAPTION= <scene name='initialview01'>1ur6</scene>
+The line below this paragraph, containing "STRUCTURE_1ur6", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ur6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ur6 OCA], [http://www.ebi.ac.uk/pdbsum/1ur6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ur6 RCSB]</span>
-}}
 '''NMR BASED STRUCTURAL MODEL OF THE UBCH5B-CNOT4 COMPLEX'''
@@ Line 32: / Line 29: @@
 [[Category: Timmers, H Th M.]]
 [[Category: Winkler, G S.]]
-[[Category: ccr4-not complex]]
+[[Category: Ccr4-not complex]]
-[[Category: ligase]]
+[[Category: Ligase]]
-[[Category: ring finger protein]]
+[[Category: Ring finger protein]]
-[[Category: transcription regulation]]
+[[Category: Transcription regulation]]
-[[Category: ubiquitin conjugating enzyme]]
+[[Category: Ubiquitin conjugating enzyme]]
-[[Category: ubiquitin ligase]]
+[[Category: Ubiquitin ligase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:34:37 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:14:02 2008''