1un9: Difference between revisions

Revision as of 11:27, 3 May 2008

CRYSTAL STRUCTURE OF THE DIHYDROXYACETONE KINASE FROM C. FREUNDII IN COMPLEX WITH AMP-PNP AND MG2+

OverviewOverview

Dihydroxyacetone kinases are a sequence-conserved family of enzymes, which utilize two different phosphoryldonors, ATP in animals, plants and some bacteria, and a multiphosphoprotein of the phosphoenolpyruvate carbohydrate phosphotransferase system in bacteria. Here we report the 2.5-A crystal structure of the homodimeric Citrobacter freundii dihydroxyacetone kinase complex with an ATP analogue and dihydroxyacetone. The N-terminal domain consists of two alpha/beta-folds with a molecule of dihydroxyacetone covalently bound in hemiaminal linkage to the N epsilon 2 of His-220. The C-terminal domain consists of a regular eight-helix alpha-barrel. The eight helices form a deep pocket, which includes a tightly bound phospholipid. Only the lipid headgroup protrudes from the surface. The nucleotide is bound on the top of the barrel across from the entrance to the lipid pocket. The phosphate groups are coordinated by two Mg2+ ions to gamma-carboxyl groups of aspartyl residues. The ATP binding site does not contain positively charged or aromatic groups. Paralogues of dihydroxyacetone kinase also occur in association with transcription regulators and proteins of unknown function pointing to biological roles beyond triose metabolism.

About this StructureAbout this Structure

1UN9 is a Single protein structure of sequence from Citrobacter freundii. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the Citrobacter freundii dihydroxyacetone kinase reveals an eight-stranded alpha-helical barrel ATP-binding domain., Siebold C, Arnold I, Garcia-Alles LF, Baumann U, Erni B, J Biol Chem. 2003 Nov 28;278(48):48236-44. Epub 2003 Sep 9. PMID:12966101 Page seeded by OCA on Sat May 3 11:27:28 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1un9.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1un9 |SIZE=350|CAPTION= <scene name='initialview01'>1un9</scene>, resolution 3.1&Aring;
+The line below this paragraph, containing "STRUCTURE_1un9", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:2ha+Binding+Site+For+Chain+B'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=2HA:DIHYDROXYACETONE'>2HA</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycerone_kinase Glycerone kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.29 2.7.1.29] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1un9|  PDB=1un9  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1un9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1un9 OCA], [http://www.ebi.ac.uk/pdbsum/1un9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1un9 RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE DIHYDROXYACETONE KINASE FROM C. FREUNDII IN COMPLEX WITH AMP-PNP AND MG2+'''
@@ Line 31: / Line 28: @@
 [[Category: Garcia-Alles, L F.]]
 [[Category: Siebold, C.]]
-[[Category: dha kinase]]
+[[Category: Dha kinase]]
-[[Category: dihydroxyacetone kinase]]
+[[Category: Dihydroxyacetone kinase]]
-[[Category: kinase,glycerone kinase]]
+[[Category: Kinase,glycerone kinase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:27:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:12:37 2008''