1ued: Difference between revisions

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Crystal Structure of OxyC a Cytochrome P450 Implicated in an Oxidative C-C Coupling Reaction During Vancomycin Biosynthesis.

OverviewOverview

Gene inactivation studies point to the involvement of OxyC in catalyzing the last oxidative phenol coupling reaction during glycopeptide antibiotic biosynthesis. Presently, the substrate and exact timing of the OxyC reaction are unknown. The substrate might be the bicyclic heptapeptide or a thioester derivative bound to a protein carrier domain. OxyC from the vancomycin producer Amycolatopsis orientalis was produced in Escherichia coli and crystallized, and its structure was determined to 1.9 A resolution. OxyC gave UV-visible spectra characteristic of a P450-like hemoprotein in the low spin ferric state. After reduction to the ferrous state by dithionite the CO-ligated form gave a 450-nm peak in a UV-difference spectrum. The addition of vancomycin aglycone to OxyC produced type I changes to the UV spectrum. OxyC exhibits the typical P450-fold, with the Cys ligand loop containing the signature sequence FGHGX-HXCLG and Cys-356 being the proximal axial thiolate ligand of the heme iron. The observation of a water molecule bound to the heme iron is consistent with the UV-visible spectra of OxyC indicating a low spin heme. A polyethylene glycol molecule occupying the active site might mimic the bicyclic heptapeptide substrate. Analysis of the structure of Oxy-proteins and other P450s indicates regions that might be involved in binding of the redox partner and possibly the protein carrier domain.

About this StructureAbout this Structure

1UED is a Single protein structure of sequence from Amycolatopsis orientalis. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of OxyC, a cytochrome P450 implicated in an oxidative C-C coupling reaction during vancomycin biosynthesis., Pylypenko O, Vitali F, Zerbe K, Robinson JA, Schlichting I, J Biol Chem. 2003 Nov 21;278(47):46727-33. Epub 2003 Jul 29. PMID:12888556 Page seeded by OCA on Sat May 3 11:06:21 2008

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 '''Crystal Structure of OxyC a Cytochrome P450 Implicated in an Oxidative C-C Coupling Reaction During Vancomycin Biosynthesis.'''
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 [[Category: Vitali, F.]]
 [[Category: Zerbe, K.]]
-[[Category: cytochrome p450 vancomycin biosynthesis]]
+[[Category: Cytochrome p450 vancomycin biosynthesis]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:06:21 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:09:11 2008''