9got: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
Line 1: Line 1:
'''Unreleased structure'''


The entry 9got is ON HOLD  until sometime in the future
==Partial (48mer) encapsulin shell assembly from Mycobacterium tuberculosis==
 
<StructureSection load='9got' size='340' side='right'caption='[[9got]], [[Resolution|resolution]] 5.42&Aring;' scene=''>
Authors: Lewis, C.J., Berger, C., Ravelli, R.B.G.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[9got]] is a 48 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GOT FirstGlance]. <br>
Description: Partial (48mer) encapsulin shell assembly from Mycobacterium tuberculosis
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.42&#8491;</td></tr>
[[Category: Unreleased Structures]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9got FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9got OCA], [https://pdbe.org/9got PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9got RCSB], [https://www.ebi.ac.uk/pdbsum/9got PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9got ProSAT]</span></td></tr>
[[Category: Ravelli, R.B.G]]
</table>
[[Category: Berger, C]]
== Function ==
[[Category: Lewis, C.J]]
[https://www.uniprot.org/uniprot/ENCAP_MYCTU ENCAP_MYCTU] Shell component of a type 1 encapsulin nanocompartment in situ; its cargo protects against oxidative stress at low pH. In situ and in E.coli assembles into proteinaceous shells about 22 nm in diameter with 2.5 nm thick walls (PubMed:24855650, PubMed:34751132). Cargo proteins are targeted to the interior via their C-terminal extensions; empty intact shells can be isolated in E.coli in the absence of cargo protein. There are at least 4 possible cargo proteins, DyP (encoded in the same locus), FolB, BfrB and Rv1762c; DyP and Rv1762c have been identified in vivo (PubMed:24855650). Probably involved in protection against oxidative damage from the host immune response (Probable) (PubMed:34751132). A T-cell antigen found in bacterial culture cell filtrates, stimulates mouse immune response. Does not have detectable bacteriocin activity (PubMed:9596740).<ref>PMID:24855650</ref> <ref>PMID:34751132</ref> <ref>PMID:9596740</ref> <ref>PMID:24855650</ref>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Berger C]]
[[Category: Lewis CJ]]
[[Category: Ravelli RBG]]

Latest revision as of 09:29, 5 February 2025

Partial (48mer) encapsulin shell assembly from Mycobacterium tuberculosisPartial (48mer) encapsulin shell assembly from Mycobacterium tuberculosis

Structural highlights

9got is a 48 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 5.42Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENCAP_MYCTU Shell component of a type 1 encapsulin nanocompartment in situ; its cargo protects against oxidative stress at low pH. In situ and in E.coli assembles into proteinaceous shells about 22 nm in diameter with 2.5 nm thick walls (PubMed:24855650, PubMed:34751132). Cargo proteins are targeted to the interior via their C-terminal extensions; empty intact shells can be isolated in E.coli in the absence of cargo protein. There are at least 4 possible cargo proteins, DyP (encoded in the same locus), FolB, BfrB and Rv1762c; DyP and Rv1762c have been identified in vivo (PubMed:24855650). Probably involved in protection against oxidative damage from the host immune response (Probable) (PubMed:34751132). A T-cell antigen found in bacterial culture cell filtrates, stimulates mouse immune response. Does not have detectable bacteriocin activity (PubMed:9596740).[1] [2] [3] [4]

References

  1. ↑ Contreras H, Joens MS, McMath LM, Le VP, Tullius MV, Kimmey JM, Bionghi N, Horwitz MA, Fitzpatrick JA, Goulding CW. Characterization of a Mycobacterium tuberculosis nanocompartment and its potential cargo proteins. J Biol Chem. 2014 Jun 27;289(26):18279-89. doi: 10.1074/jbc.M114.570119. Epub, 2014 May 22. PMID:24855650 doi:http://dx.doi.org/10.1074/jbc.M114.570119
  2. ↑ Lien KA, Dinshaw K, Nichols RJ, Cassidy-Amstutz C, Knight M, Singh R, Eltis LD, Savage DF, Stanley SA. A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis. Elife. 2021 Nov 9;10. pii: 74358. doi: 10.7554/eLife.74358. PMID:34751132 doi:http://dx.doi.org/10.7554/eLife.74358
  3. ↑ Rosenkrands I, Rasmussen PB, Carnio M, Jacobsen S, Theisen M, Andersen P. Identification and characterization of a 29-kilodalton protein from Mycobacterium tuberculosis culture filtrate recognized by mouse memory effector cells. Infect Immun. 1998 Jun;66(6):2728-35. doi: 10.1128/IAI.66.6.2728-2735.1998. PMID:9596740 doi:http://dx.doi.org/10.1128/IAI.66.6.2728-2735.1998
  4. ↑ Contreras H, Joens MS, McMath LM, Le VP, Tullius MV, Kimmey JM, Bionghi N, Horwitz MA, Fitzpatrick JA, Goulding CW. Characterization of a Mycobacterium tuberculosis nanocompartment and its potential cargo proteins. J Biol Chem. 2014 Jun 27;289(26):18279-89. doi: 10.1074/jbc.M114.570119. Epub, 2014 May 22. PMID:24855650 doi:http://dx.doi.org/10.1074/jbc.M114.570119

9got, resolution 5.42Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=9got&oldid=4253132"