1u0f: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1u0f.jpg|left|200px]] | [[Image:1u0f.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1u0f", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
--> | |||
{{STRUCTURE_1u0f| PDB=1u0f | SCENE= }} | |||
}} | |||
'''Crystal structure of mouse phosphoglucose isomerase in complex with glucose 6-phosphate''' | '''Crystal structure of mouse phosphoglucose isomerase in complex with glucose 6-phosphate''' | ||
| Line 35: | Line 32: | ||
[[Category: Swan, M K.]] | [[Category: Swan, M K.]] | ||
[[Category: Zimmerly, E M.]] | [[Category: Zimmerly, E M.]] | ||
[[Category: | [[Category: Aldose-ketose isomerase]] | ||
[[Category: | [[Category: Dimer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:35:45 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 10:35, 3 May 2008
Crystal structure of mouse phosphoglucose isomerase in complex with glucose 6-phosphate
OverviewOverview
Phosphoglucose isomerase (PGI) is an enzyme of glycolysis that interconverts glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) but, outside the cell, is a multifunctional cytokine. High-resolution crystal structures of the enzyme from mouse have been determined in native form and in complex with the inhibitor erythrose 4-phosphate, and with the substrate glucose 6-phosphate. In the substrate-bound structure, the glucose sugar is observed in both straight-chain and ring forms. This structure supports a specific role for Lys518 in enzyme-catalyzed ring opening and we present a "push-pull" mechanism in which His388 breaks the O5-C1 bond by donating a proton to the ring oxygen atom and, simultaneously, Lys518 abstracts a proton from the C1 hydroxyl group. The reverse occurs in ring closure. The transition from ring form to straight-chain substrate is achieved through rotation of the C3-C4 bond, which brings the C1-C2 region into close proximity to Glu357, the base catalyst for the isomerization step. The structure with G6P also explains the specificity of PGI for glucose 6-phosphate over mannose 6-isomerase (M6P). To isomerize M6P to F6P requires a rotation of its C2-C3 bond but in PGI this is sterically blocked by Gln511.
About this StructureAbout this Structure
1U0F is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of mouse phosphoglucose isomerase at 1.6A resolution and its complex with glucose 6-phosphate reveals the catalytic mechanism of sugar ring opening., Graham Solomons JT, Zimmerly EM, Burns S, Krishnamurthy N, Swan MK, Krings S, Muirhead H, Chirgwin J, Davies C, J Mol Biol. 2004 Sep 17;342(3):847-60. PMID:15342241 Page seeded by OCA on Sat May 3 10:35:45 2008