8y9e: Difference between revisions

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-'''Unreleased structure'''
-The entry 8y9e is ON HOLD  until Paper Publication
+==Versatile Aromatic Prenyltransferase auraA for Imidazole-Containing Diketopiperazines==
+<StructureSection load='8y9e' size='340' side='right'caption='[[8y9e]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8y9e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium Penicillium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Y9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Y9E FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8y9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8y9e OCA], [https://pdbe.org/8y9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8y9e RCSB], [https://www.ebi.ac.uk/pdbsum/8y9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8y9e ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Prenylation modifications of natural products play essential roles in chemical diversity and bioactivities, but imidazole modification prenyltransferases are not well investigated. Here, we discover a dimethylallyl tryptophan synthase family prenyltransferase, AuraA, that catalyzes the rare dimethylallylation on the imidazole moiety in the biosynthesis of aurantiamine. Biochemical assays validate that AuraA could accept both cyclo-(L-Val-L-His) and cyclo-(L-Val-DH-His) as substrates, while the prenylation modes are completely different, yielding C2-regular and C5-reverse products, respectively. Cryo-electron microscopy analysis of AuraA and its two ternary complex structures reveal two distinct modes for receptor binding, demonstrating a tolerance for altered orientations of highly similar receptors. The mutation experiments further demonstrate the promiscuity of AuraA towards imidazole-C-dimethylallylation. In this work, we also characterize a case of AuraA mutant-catalyzed dimethylallylation of imidazole moiety, offering available structural insights into the utilization and engineering of dimethylallyl tryptophan synthase family prenyltransferases.
-Authors: Dehai, L., Yuzhong, Z., Wenxue, W., Peng, W.
+Characterization and structural analysis of a versatile aromatic prenyltransferase for imidazole-containing diketopiperazines.,Wang W, Wang P, Ma C, Li K, Wang Z, Liu Y, Wang L, Zhang G, Che Q, Zhu T, Zhang Y, Li D Nat Commun. 2025 Jan 2;16(1):144. doi: 10.1038/s41467-024-55537-8. PMID:39747040<ref>PMID:39747040</ref>
-Description: Versatile Aromatic Prenyltransferase auraA for Imidazole-Containing Diketopiperazines
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Wenxue, W]]
+<div class="pdbe-citations 8y9e" style="background-color:#fffaf0;"></div>
-[[Category: Yuzhong, Z]]
+== References ==
-[[Category: Peng, W]]
+<references/>
-[[Category: Dehai, L]]
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Penicillium]]
+[[Category: Li D]]
+[[Category: Wang P]]
+[[Category: Wang W]]
+[[Category: Zhang Y]]