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==Comamonas testosteroni KF-1 circularly permuted group II intron Post-2S state== | |||
<StructureSection load='8xtp' size='340' side='right'caption='[[8xtp]], [[Resolution|resolution]] 2.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8xtp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Comamonas_testosteroni_KF-1 Comamonas testosteroni KF-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XTP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xtp OCA], [https://pdbe.org/8xtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xtp RCSB], [https://www.ebi.ac.uk/pdbsum/8xtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xtp ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Circularly permuted group II introns (CP introns) consist of rearranged structural domains separated by two tethered exons, generating branched introns and circular exons via back-splicing. Structural and mechanistic understanding of circular RNA (circRNA) generation by CP introns remains elusive. We resolve cryo-electron microscopy structures of a natural CP intron in different states during back-splicing at a resolution of 2.5-2.9 A. Domain 6 (D6) undergoes a conformational change of 65 degrees after branching, to facilitate 3'-exon recognition and circularization. Previously unseen tertiary interactions compact the catalytic triad and D6 for splicing without protein, whereas a metal ion, M(35), is observed to stabilize the 5'-exon during splicing. While these unique features were not observed in canonical group II introns and spliceosomes, they are common in CP introns, as demonstrated by the cryo-EM structure of another CP intron discovered by comparative genomics analysis. Our results elucidate the mechanism of CP intron back-splicing dynamics, with potential applications in circRNA research and therapeutics. | |||
Structural basis of circularly permuted group II intron self-splicing.,Wang L, Xie J, Zhang C, Zou J, Huang Z, Shang S, Chen X, Yang Y, Liu J, Dong H, Huang D, Su Z Nat Struct Mol Biol. 2025 Jan 31. doi: 10.1038/s41594-025-01484-x. PMID:39890981<ref>PMID:39890981</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8xtp" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Huang | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Comamonas testosteroni KF-1]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Chen XY]] | ||
[[Category: Yang | [[Category: Dong HH]] | ||
[[Category: Zhang | [[Category: Huang DM]] | ||
[[Category: | [[Category: Huang ZR]] | ||
[[Category: Liu J]] | |||
[[Category: Shang ST]] | |||
[[Category: Su ZM]] | |||
[[Category: Wang L]] | |||
[[Category: Xie JH]] | |||
[[Category: Yang Y]] | |||
[[Category: Zhang C]] | |||
[[Category: Zou J]] | |||