8x7f: Difference between revisions
Jump to navigation
Jump to search
m Protected "8x7f" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
==CryoEM structure of the trifunctional NAD biosynthesis/regulator protein NadR in complex with NAD== | |||
<StructureSection load='8x7f' size='340' side='right'caption='[[8x7f]], [[Resolution|resolution]] 2.66Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8x7f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8X7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8X7F FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.66Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8x7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8x7f OCA], [https://pdbe.org/8x7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8x7f RCSB], [https://www.ebi.ac.uk/pdbsum/8x7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8x7f ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NADR_ECOLI NADR_ECOLI] This enzyme has three activities: DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase within the cell, the affinity of NadR for the nadB operator regions of nadA, nadB, and pncB increases, repressing the transcription of these genes. The RN kinase activity catalyzes the phosphorylation of RN to form nicotinamide ribonucleotide. The NMN adenylyltransferase activity catalyzes the transfer of the AMP moiety of ATP to nicotinamide ribonucleotide to form NAD(+). The NMN adenylyltransferase domain also functions as the NAD and ATP sensor.<ref>PMID:10464228</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | |||
[[Category: Cheng XQ]] | |||
[[Category: Dong X]] | |||
[[Category: Jiang WX]] | |||
[[Category: Ma LX]] | |||
[[Category: Xing Q]] | |||
Latest revision as of 09:16, 4 December 2024
CryoEM structure of the trifunctional NAD biosynthesis/regulator protein NadR in complex with NADCryoEM structure of the trifunctional NAD biosynthesis/regulator protein NadR in complex with NAD
Structural highlights
FunctionNADR_ECOLI This enzyme has three activities: DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase within the cell, the affinity of NadR for the nadB operator regions of nadA, nadB, and pncB increases, repressing the transcription of these genes. The RN kinase activity catalyzes the phosphorylation of RN to form nicotinamide ribonucleotide. The NMN adenylyltransferase activity catalyzes the transfer of the AMP moiety of ATP to nicotinamide ribonucleotide to form NAD(+). The NMN adenylyltransferase domain also functions as the NAD and ATP sensor.[1] References
|
| ||||||||||||||||||