1twc: Difference between revisions

Revision as of 10:27, 3 May 2008

RNA polymerase II complexed with GTP

OverviewOverview

Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a mismatched nucleoside triphosphate was also detected, but in an adjacent site, inverted with respect to the correctly paired nucleotide. The results are consistent with a two-step mechanism of nucleotide selection, with initial binding to an entry (E) site beneath the active center in an inverted orientation, followed by rotation into the nucleotide addition (A) site for pairing with the template DNA. This mechanism is unrelated to that of single subunit RNA polymerases and so defines a new paradigm for the large, multisubunit enzymes. Additional findings from these studies include a third nucleotide binding site that may define the length of backtracked RNA; DNA double helix unwinding in advance of the polymerase active center; and extension of the diffraction limit of RNA polymerase II crystals to 2.3 A.

About this StructureAbout this Structure

1TWC is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center., Westover KD, Bushnell DA, Kornberg RD, Cell. 2004 Nov 12;119(4):481-9. PMID:15537538 Page seeded by OCA on Sat May 3 10:27:06 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1twc.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1twc |SIZE=350|CAPTION= <scene name='initialview01'>1twc</scene>, resolution 3.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1twc", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1twc|  PDB=1twc  |  SCENE=  }}
-|RELATEDENTRY=[[1twa|1TWA]], [[1twf|1TWF]], [[1twg|1TWG]], [[1twh|1TWH]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twc OCA], [http://www.ebi.ac.uk/pdbsum/1twc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1twc RCSB]</span>
-}}
 '''RNA polymerase II complexed with GTP'''
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 [[Category: Kornberg, R D.]]
 [[Category: Westover, K D.]]
-[[Category: molecular machine]]
+[[Category: Molecular machine]]
-[[Category: mrna]]
+[[Category: Mrna]]
-[[Category: multiprotein complex]]
+[[Category: Multiprotein complex]]
-[[Category: transcription]]
+[[Category: Transcription]]
-[[Category: zinc motif]]
+[[Category: Zinc motif]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:27:06 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:02:04 2008''