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==D10N,P146A variant of beta-phosphoglucomutase from Lactococcus lactis in complex with native beta-glucose 1,6-bisphosphate intermediate== | |||
<StructureSection load='8q1f' size='340' side='right'caption='[[8q1f]], [[Resolution|resolution]] 1.01Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8q1f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_Il1403 Lactococcus lactis subsp. lactis Il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Q1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Q1F FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.01Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B16:1,6-DI-O-PHOSPHONO-BETA-D-GLUCOPYRANOSE'>B16</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8q1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8q1f OCA], [https://pdbe.org/8q1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8q1f RCSB], [https://www.ebi.ac.uk/pdbsum/8q1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8q1f ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0A7T4I1_LACLL A0A0A7T4I1_LACLL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Metabolic regulation occurs through precise control of enzyme activity. Allomorphy is a post-translational fine control mechanism where the catalytic rate is governed by a conformational switch that shifts the enzyme population between forms with different activities. beta-Phosphoglucomutase (betaPGM) uses allomorphy in the catalysis of isomerisation of beta-glucose 1-phosphate to glucose 6-phosphate via beta-glucose 1,6-bisphosphate. Herein, we describe structural and biophysical approaches to reveal its allomorphic regulatory mechanism. Binding of the full allomorphic activator beta-glucose 1,6-bisphosphate stimulates enzyme closure, progressing through NAC I and NAC III conformers. Prior to phosphoryl transfer, loops positioned on the cap and core domains are brought into close proximity, modulating the environment of a key proline residue. Hence accelerated isomerisation, likely via a twisted anti/C4-endo transition state, leads to the rapid predominance of active cis-P betaPGM. In contrast, binding of the partial allomorphic activator fructose 1,6-bisphosphate arrests betaPGM at a NAC I conformation and phosphoryl transfer to both cis-P betaPGM and trans-P betaPGM occurs slowly. Thus, allomorphy allows a rapid response to changes in food supply while not otherwise impacting substantially on levels of important metabolites. | |||
Peri active site catalysis of proline isomerisation is the molecular basis of allomorphy in beta-phosphoglucomutase.,Cruz-Navarrete FA, Baxter NJ, Flinders AJ, Buzoianu A, Cliff MJ, Baker PJ, Waltho JP Commun Biol. 2024 Jul 27;7(1):909. doi: 10.1038/s42003-024-06577-9. PMID:39068257<ref>PMID:39068257</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8q1f" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lactococcus lactis subsp. lactis Il1403]] | |||
[[Category: Large Structures]] | |||
[[Category: Baker PJ]] | |||
[[Category: Baxter NJ]] | |||
[[Category: Buzoianu A]] | |||
[[Category: Cliff MJ]] | |||
[[Category: Cruz-Navarrete FA]] | |||
[[Category: Flinders AJ]] | |||
[[Category: Waltho JP]] | |||