1ttt: Difference between revisions

Revision as of 10:21, 3 May 2008

PHE-TRNA, ELONGATION FACTOR EF-TU:GDPNP TERNARY COMPLEX

OverviewOverview

The structure of the ternary complex consisting of yeast phenylalanyl-transfer RNA (Phe-tRNAPhe), Thermus aquaticus elongation factor Tu (EF-Tu), and the guanosine triphosphate (GTP) analog GDPNP was determined by x-ray crystallography at 2.7 angstrom resolution. The ternary complex participates in placing the amino acids in their correct order when messenger RNA is translated into a protein sequence on the ribosome. The EF-Tu-GDPNP component binds to one side of the acceptor helix of Phe-tRNAPhe involving all three domains of EF-Tu. Binding sites for the phenylalanylated CCA end and the phosphorylated 5' end are located at domain interfaces, whereas the T stem interacts with the surface of the beta-barrel domain 3. The binding involves many conserved residues in EF-Tu. The overall shape of the ternary complex is similar to that of the translocation factor, EF-G-GDP, and this suggests a novel mechanism involving "molecular mimicry" in the translational apparatus.

About this StructureAbout this Structure

1TTT is a Single protein structure of sequence from Thermus aquaticus. The following page contains interesting information on the relation of 1TTT with [Elongation Factors]. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the ternary complex of Phe-tRNAPhe, EF-Tu, and a GTP analog., Nissen P, Kjeldgaard M, Thirup S, Polekhina G, Reshetnikova L, Clark BF, Nyborg J, Science. 1995 Dec 1;270(5241):1464-72. PMID:7491491 Page seeded by OCA on Sat May 3 10:21:17 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ttt.jpg|left|200px]]
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ttt OCA], [http://www.ebi.ac.uk/pdbsum/1ttt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ttt RCSB]</span>
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 '''PHE-TRNA, ELONGATION FACTOR EF-TU:GDPNP TERNARY COMPLEX'''
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 [[Category: 1eft]]
 [[Category: 4tna]]
-[[Category: complex (elongation factor/trna)]]
+[[Category: Ef-tu]]
-[[Category: ef-tu]]
+[[Category: Peptide elongation ribonucleoprotein]]
-[[Category: peptide elongation ribonucleoprotein]]
+[[Category: Protein synthesis]]
-[[Category: protein synthesis]]
+[[Category: Ribosome]]
-[[Category: ribosome]]
+[[Category: Trna]]
-[[Category: trna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:21:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:00:57 2008''