7yr8: Difference between revisions
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<StructureSection load='7yr8' size='340' side='right'caption='[[7yr8]], [[Resolution|resolution]] 3.20Å' scene=''> | <StructureSection load='7yr8' size='340' side='right'caption='[[7yr8]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7yr8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[7yr8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cuevavirus_lloviuense Cuevavirus lloviuense] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YR8 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yr8 OCA], [https://pdbe.org/7yr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yr8 RCSB], [https://www.ebi.ac.uk/pdbsum/7yr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yr8 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yr8 OCA], [https://pdbe.org/7yr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yr8 RCSB], [https://www.ebi.ac.uk/pdbsum/7yr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yr8 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/G8EFI1_LLOVA G8EFI1_LLOVA] | [https://www.uniprot.org/uniprot/G8EFI1_LLOVA G8EFI1_LLOVA] | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lloviu virus (LLOV) is a novel filovirus detected in Schreiber's bats in Europe. The isolation of the infectious LLOV from bats has raised public health concerns. However, the virological and molecular characteristics of LLOV remain largely unknown. The nucleoprotein (NP) of LLOV encapsidates the viral genomic RNA to form a helical NP-RNA complex, which acts as a scaffold for nucleocapsid formation and de novo viral RNA synthesis. In this study, using single-particle cryoelectron microscopy, we determined two structures of the LLOV NP-RNA helical complex, comprising a full-length and a C-terminally truncated NP. The two helical structures were identical, demonstrating that the N-terminal region determines the helical arrangement of the NP. The LLOV NP-RNA protomers displayed a structure similar to that in the Ebola and Marburg virus, but the spatial arrangements in the helix differed. Structure-based mutational analysis identified amino acids involved in the helical assembly and viral RNA synthesis. These structures advance our understanding of the filovirus nucleocapsid formation and provide a structural basis for the development of antifiloviral therapeutics. | |||
Cryoelectron microscopic structure of the nucleoprotein-RNA complex of the European filovirus, Lloviu virus.,Hu S, Fujita-Fujiharu Y, Sugita Y, Wendt L, Muramoto Y, Nakano M, Hoenen T, Noda T PNAS Nexus. 2023 Apr 6;2(4):pgad120. doi: 10.1093/pnasnexus/pgad120. eCollection , 2023 Apr. PMID:37124400<ref>PMID:37124400</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7yr8" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Cuevavirus lloviuense]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Synthetic construct]] | [[Category: Synthetic construct]] | ||
[[Category: Fujita-Fujiharu Y]] | [[Category: Fujita-Fujiharu Y]] | ||
Latest revision as of 10:34, 3 July 2024
Lloviu cuevavirus nucleoprotein(1-450 residues)-RNA complexLloviu cuevavirus nucleoprotein(1-450 residues)-RNA complex
Structural highlights
FunctionPublication Abstract from PubMedLloviu virus (LLOV) is a novel filovirus detected in Schreiber's bats in Europe. The isolation of the infectious LLOV from bats has raised public health concerns. However, the virological and molecular characteristics of LLOV remain largely unknown. The nucleoprotein (NP) of LLOV encapsidates the viral genomic RNA to form a helical NP-RNA complex, which acts as a scaffold for nucleocapsid formation and de novo viral RNA synthesis. In this study, using single-particle cryoelectron microscopy, we determined two structures of the LLOV NP-RNA helical complex, comprising a full-length and a C-terminally truncated NP. The two helical structures were identical, demonstrating that the N-terminal region determines the helical arrangement of the NP. The LLOV NP-RNA protomers displayed a structure similar to that in the Ebola and Marburg virus, but the spatial arrangements in the helix differed. Structure-based mutational analysis identified amino acids involved in the helical assembly and viral RNA synthesis. These structures advance our understanding of the filovirus nucleocapsid formation and provide a structural basis for the development of antifiloviral therapeutics. Cryoelectron microscopic structure of the nucleoprotein-RNA complex of the European filovirus, Lloviu virus.,Hu S, Fujita-Fujiharu Y, Sugita Y, Wendt L, Muramoto Y, Nakano M, Hoenen T, Noda T PNAS Nexus. 2023 Apr 6;2(4):pgad120. doi: 10.1093/pnasnexus/pgad120. eCollection , 2023 Apr. PMID:37124400[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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