8cxp: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8cxp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Senecavirus_A Senecavirus A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CXP FirstGlance]. <br> | <table><tr><td colspan='2'>[[8cxp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Senecavirus_A Senecavirus A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CXP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cxp OCA], [https://pdbe.org/8cxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cxp RCSB], [https://www.ebi.ac.uk/pdbsum/8cxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cxp ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.47Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cxp OCA], [https://pdbe.org/8cxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cxp RCSB], [https://www.ebi.ac.uk/pdbsum/8cxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cxp ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
Latest revision as of 08:18, 12 June 2024
Characterisation of a Seneca Valley Virus Thermostable MutantCharacterisation of a Seneca Valley Virus Thermostable Mutant
Structural highlights
Publication Abstract from PubMedSeneca Valley virus (SVV) is a newly discovered picornavirus in the Senecavirus genus. SVV-001 strain has shown promise as an oncolytic virus against tumors with neuroendocrine features. There is a need to use a structure-based approach to develop virus-like particles capable to mimicking the architecture of naturally occurring empty capsids that can be used as vaccines or as carriers for targeted cancer treatment. However, these empty capsids are inherently less stable, and tedious to purify. This warrants investigation into factors which confer the SVV capsid stability and into combining this knowledge to recombinantly express stable SVV VLPs. In this study, we isolated a thermostable mutant of SVV by thermal selection assays and we characterized a single mutation located in a capsid protein. The cryo-EM map of this mutant showed conformational shifts that facilitated the formation of additional hydrogen bonds and aromatic interactions, which could serve as capsid stabilizing factors. Characterisation of a Seneca Valley virus thermostable mutant.,Jayawardena N, McCarthy C, Wang I, Waqqar S, Burga LN, Strauss M, Bostina M Virology. 2022 Oct;575:74-82. doi: 10.1016/j.virol.2022.08.015. Epub 2022 Aug 30. PMID:36084546[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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