7xtd: Difference between revisions
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<StructureSection load='7xtd' size='340' side='right'caption='[[7xtd]], [[Resolution|resolution]] 3.90Å' scene=''> | <StructureSection load='7xtd' size='340' side='right'caption='[[7xtd]], [[Resolution|resolution]] 3.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7xtd]] is a | <table><tr><td colspan='2'>[[7xtd]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Komagataella_phaffii Komagataella phaffii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XTD FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xtd OCA], [https://pdbe.org/7xtd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xtd RCSB], [https://www.ebi.ac.uk/pdbsum/7xtd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xtd ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xtd OCA], [https://pdbe.org/7xtd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xtd RCSB], [https://www.ebi.ac.uk/pdbsum/7xtd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xtd ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/ | [https://www.uniprot.org/uniprot/C4R3K1_KOMPG C4R3K1_KOMPG] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but | During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but the mechanism has remained elusive. Using cryo-electron microscopy, we obtained structures of the RNAPII elongation complex (EC) passing through a nucleosome in the presence of the transcription elongation factors Spt6, Spn1, Elf1, Spt4/5, and Paf1C and the histone chaperone FACT (facilitates chromatin transcription). The structures show snapshots of EC progression on DNA mediating downstream nucleosome disassembly, followed by its reassembly upstream of the EC, which is facilitated by FACT. FACT dynamically adapts to successively occurring subnucleosome intermediates, forming an interface with the EC. Spt6, Spt4/5, and Paf1C form a "cradle" at the EC DNA-exit site and support the upstream nucleosome reassembly. These structures explain the mechanism by which the EC traverses nucleosomes while maintaining the chromatin structure and epigenetic information. | ||
Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT.,Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI Science. 2022 | Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT.,Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI Science. 2022 Sep 9;377(6611):eabp9466. doi: 10.1126/science.abp9466. Epub 2022 , Aug 18. PMID:35981082<ref>PMID:35981082</ref> | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 7xtd" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 7xtd" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Komagataella phaffii]] | [[Category: Komagataella phaffii]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Synthetic construct]] | |||
[[Category: Ehara H]] | [[Category: Ehara H]] | ||
[[Category: Kujirai T]] | [[Category: Kujirai T]] | ||
Latest revision as of 10:29, 3 July 2024
RNA polymerase II elongation complex transcribing a nucleosome (EC58oct)RNA polymerase II elongation complex transcribing a nucleosome (EC58oct)
Structural highlights
FunctionPublication Abstract from PubMedDuring gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but the mechanism has remained elusive. Using cryo-electron microscopy, we obtained structures of the RNAPII elongation complex (EC) passing through a nucleosome in the presence of the transcription elongation factors Spt6, Spn1, Elf1, Spt4/5, and Paf1C and the histone chaperone FACT (facilitates chromatin transcription). The structures show snapshots of EC progression on DNA mediating downstream nucleosome disassembly, followed by its reassembly upstream of the EC, which is facilitated by FACT. FACT dynamically adapts to successively occurring subnucleosome intermediates, forming an interface with the EC. Spt6, Spt4/5, and Paf1C form a "cradle" at the EC DNA-exit site and support the upstream nucleosome reassembly. These structures explain the mechanism by which the EC traverses nucleosomes while maintaining the chromatin structure and epigenetic information. Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT.,Ehara H, Kujirai T, Shirouzu M, Kurumizaka H, Sekine SI Science. 2022 Sep 9;377(6611):eabp9466. doi: 10.1126/science.abp9466. Epub 2022 , Aug 18. PMID:35981082[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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