7zox: Difference between revisions
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==Nup93 in complex with | ==Nup93 in complex with xhNup93-Nb4i and xNup93-Nb2t== | ||
<StructureSection load='7zox' size='340' side='right'caption='[[7zox]], [[Resolution|resolution]] 4.40Å' scene=''> | <StructureSection load='7zox' size='340' side='right'caption='[[7zox]], [[Resolution|resolution]] 4.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/NUP93_XENLA NUP93_XENLA] Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. | [https://www.uniprot.org/uniprot/NUP93_XENLA NUP93_XENLA] Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nuclear pore complex (NPC) biogenesis is a still enigmatic example of protein self-assembly. We now introduce several cross-reacting anti-Nup nanobodies for imaging intact nuclear pore complexes from frog to human. We also report a simplified assay that directly tracks postmitotic NPC assembly with added fluorophore-labeled anti-Nup nanobodies. During interphase, NPCs are inserted into a pre-existing nuclear envelope. Monitoring this process is challenging because newly assembled NPCs are indistinguishable from pre-existing ones. We overcame this problem by inserting Xenopus-derived NPCs into human nuclear envelopes and using frog-specific anti-Nup nanobodies for detection. We further asked whether anti-Nup nanobodies could serve as NPC assembly inhibitors. Using a selection strategy against conserved epitopes, we obtained anti-Nup93, Nup98, and Nup155 nanobodies that block Nup-Nup interfaces and arrest NPC assembly. We solved structures of nanobody-target complexes and identified roles for the Nup93 alpha-solenoid domain in recruiting Nup358 and the Nup214.88.62 complex, as well as for Nup155 and the Nup98 autoproteolytic domain in NPC scaffold assembly. The latter suggests a checkpoint linking pore formation to the assembly of the Nup98-dominated permeability barrier. | |||
A checkpoint function for Nup98 in nuclear pore formation suggested by novel inhibitory nanobodies.,Sola Colom M, Fu Z, Gunkel P, Guttler T, Trakhanov S, Srinivasan V, Gregor K, Pleiner T, Gorlich D EMBO J. 2024 Jun;43(11):2198-2232. doi: 10.1038/s44318-024-00081-w. Epub 2024 Apr , 22. PMID:38649536<ref>PMID:38649536</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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<div class="pdbe-citations 7zox" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||