7n9f: Difference between revisions
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==== | ==Structure of the in situ yeast NPC== | ||
<StructureSection load='7n9f' size='340' side='right'caption='[[7n9f]]' scene=''> | <StructureSection load='7n9f' size='340' side='right'caption='[[7n9f]], [[Resolution|resolution]] 37.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7n9f]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N9F FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n9f OCA], [https://pdbe.org/7n9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n9f RCSB], [https://www.ebi.ac.uk/pdbsum/7n9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n9f ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 37Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n9f OCA], [https://pdbe.org/7n9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n9f RCSB], [https://www.ebi.ac.uk/pdbsum/7n9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n9f ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/NU170_YEAST NU170_YEAST] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP170 probably plays an important role in NPC assembly and organization. In addition it is required for chromosome transmission fidelity.<ref>PMID:9864357</ref> <ref>PMID:12403813</ref> <ref>PMID:14697200</ref> <ref>PMID:11290711</ref> <ref>PMID:12473689</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure of the isolated yeast NPC in which the inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible connectors tie together different structural and functional layers. These connectors may be targets for phosphorylation and regulated disassembly in cells with an open mitosis. Moreover, some nucleoporin pairs and transport factors have similar interaction motifs, which suggests an evolutionary and mechanistic link between assembly and transport. We provide evidence for three major NPC variants that may foreshadow functional specializations at the nuclear periphery. Cryo-electron tomography extended these studies, providing a model of the in situ NPC with a radially expanded inner ring. Our comprehensive model reveals features of the nuclear basket and central transporter, suggests a role for the lumenal Pom152 ring in restricting dilation, and highlights structural plasticity that may be required for transport. | |||
Comprehensive structure and functional adaptations of the yeast nuclear pore complex.,Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP Cell. 2022 Jan 20;185(2):361-378.e25. doi: 10.1016/j.cell.2021.12.015. Epub 2022 , Jan 3. PMID:34982960<ref>PMID:34982960</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7n9f" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nucleoporin 3D structures|Nucleoporin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Akey CW]] | |||
[[Category: Echeverria I]] | |||
[[Category: Ludtke SJ]] | |||
[[Category: Rout MP]] | |||
[[Category: Singh D]] | |||
[[Category: Suslov S]] | |||
[[Category: Villa E]] | |||