1tm5: Difference between revisions

Revision as of 10:07, 3 May 2008

crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59A mutant

OverviewOverview

A series of mutants of chymotrypsin inhibitor 2 (CI2), at residues that interact with the inhibited enzyme subtilisin BPN', were studied to determine the relative importance of intermolecular contacts on either side of the scissile bond. Mutants were tested for inhibition of subtilisin, rates of hydrolysis by subtilisin, and ability to acylate subtilisin. Additionally, crystal structures of the mutant CI2 complexes with subtilisin were obtained. Ordered water molecules were found to play an important role in inhibitor recognition, and features of the crystal structures, in combination with biochemical data, support a transition-state stabilization role for the P(1) residue in subtilisin catalysis. Consistent with the proposed mechanism of inhibition, in which rapid acylation is followed by religation, leaving-group contacts with the enzyme were found to be more critical determinants of inhibition than acylating-group contacts in the mutants studied here.

About this StructureAbout this Structure

1TM5 is a Protein complex structure of sequences from Bacillus amyloliquefaciens and Hordeum vulgare subsp. vulgare. Full crystallographic information is available from OCA.

ReferenceReference

Binding, proteolytic, and crystallographic analyses of mutations at the protease-inhibitor interface of the subtilisin BPN'/chymotrypsin inhibitor 2 complex., Radisky ES, Kwan G, Karen Lu CJ, Koshland DE Jr, Biochemistry. 2004 Nov 2;43(43):13648-56. PMID:15504027 Page seeded by OCA on Sat May 3 10:07:18 2008

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OCA

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 [[Image:1tm5.jpg|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span>
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-|DOMAIN=
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-|RELATEDENTRY=[[1tm1|1TM1]], [[1tm3|1TM3]], [[1tm4|1TM4]], [[1tm7|1TM7]], [[1tmg|1TMG]], [[1to1|1TO1]], [[1to2|1TO2]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tm5 OCA], [http://www.ebi.ac.uk/pdbsum/1tm5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tm5 RCSB]</span>
-}}
 '''crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 M59A mutant'''
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 [[Category: Lu, C J.Karen.]]
 [[Category: Radisky, E S.]]
-[[Category: inhibitor]]
+[[Category: Inhibitor]]
-[[Category: serine protease]]
+[[Category: Serine protease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:07:18 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:58:02 2008''