7ea3: Difference between revisions
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==== | ==Intact Ypt32-TRAPPII (dimer).== | ||
<StructureSection load='7ea3' size='340' side='right'caption='[[7ea3]]' scene=''> | <StructureSection load='7ea3' size='340' side='right'caption='[[7ea3]], [[Resolution|resolution]] 4.31Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ea3]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EA3 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ea3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ea3 OCA], [https://pdbe.org/7ea3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ea3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ea3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ea3 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.31Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ea3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ea3 OCA], [https://pdbe.org/7ea3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ea3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ea3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ea3 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/BET3_YEAST BET3_YEAST] Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication.<ref>PMID:8590804</ref> <ref>PMID:9564032</ref> <ref>PMID:11239471</ref> <ref>PMID:20972447</ref> <ref>PMID:20375281</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Transport protein particle (TRAPP) complexes belong to the multiprotein tethering complex and exist in three forms-core TRAPP/TRAPPI, TRAPPII, and TRAPPIII. TRAPPII activates GTPase Ypt31/Ypt32 as the guanine nucleotide exchange factor in the trans-Golgi network to determine the maturation of Golgi cisternae into post-Golgi carriers in yeast. Here, we present cryo-EM structures of yeast TRAPPII in apo and Ypt32-bound states. All the structures show a dimeric architecture assembled by two triangle-shaped monomers, while the monomer in the apo state exhibits both open and closed conformations, and the monomer in the Ypt32-bound form only captures the closed conformation. Located in the interior of the monomer, Ypt32 binds with both core TRAPP/TRAPPI and Trs120 via its nucleotide-binding domain and binds with Trs31 via its hypervariable domain. Combined with functional analysis, the structures provide insights into the assembly of TRAPPII and the mechanism of the specific activation of Ypt31/Ypt32 by TRAPPII. | |||
Structural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32.,Mi C, Zhang L, Huang G, Shao G, Yang F, You X, Dong MQ, Sun S, Sui SF Sci Adv. 2022 Jan 28;8(4):eabi5603. doi: 10.1126/sciadv.abi5603. Epub 2022 Jan , 26. PMID:35080977<ref>PMID:35080977</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ea3" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Saccharomyces cerevisiae S288C]] | ||
[[Category: Mi CC]] | |||
[[Category: Sui SF]] | |||