7ns2: Difference between revisions
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==Virion of Leishmania RNA virus 1== | ==Virion of Leishmania RNA virus 1== | ||
<StructureSection load='7ns2' size='340' side='right'caption='[[7ns2]], [[Resolution|resolution]] 3. | <StructureSection load='7ns2' size='340' side='right'caption='[[7ns2]], [[Resolution|resolution]] 3.63Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7ns2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_RNA_virus_1_-_LgM5313 Leishmania RNA virus 1 - LgM5313]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NS2 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ns2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_RNA_virus_1_-_LgM5313 Leishmania RNA virus 1 - LgM5313]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NS2 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ns2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ns2 OCA], [https://pdbe.org/7ns2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ns2 RCSB], [https://www.ebi.ac.uk/pdbsum/7ns2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ns2 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.63Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ns2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ns2 OCA], [https://pdbe.org/7ns2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ns2 RCSB], [https://www.ebi.ac.uk/pdbsum/7ns2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ns2 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/L7XUU7_9VIRU L7XUU7_9VIRU] | [https://www.uniprot.org/uniprot/L7XUU7_9VIRU L7XUU7_9VIRU] | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The presence of Leishmania RNA virus 1 (LRV1) enables Leishmania protozoan parasites to cause more severe disease than the virus-free strains. The structure of LRV1 virus-like particles has been determined previously, however, the structure of the LRV1 virion has not been characterized. Here we used cryo-electron microscopy and single-particle reconstruction to determine the structures of the LRV1 virion and empty particle isolated from Leishmania guyanensis to resolutions of 4.0 A and 3.6 A, respectively. The capsid of LRV1 is built from sixty dimers of capsid proteins organized with icosahedral symmetry. RNA genomes of totiviruses are replicated inside the virions by RNA polymerases expressed as C-terminal extensions of a sub-population of capsid proteins. Most of the virions probably contain one or two copies of the RNA polymerase, however, the location of the polymerase domains in LRV1 capsid could not be identified, indicating that it varies among particles. Importance. Every year over 200 000 people contract leishmaniasis and more than five hundred people die of the disease. The mucocutaneous form of leishmaniasis produces lesions that can destroy the mucous membranes of the nose, mouth, and throat. Leishmania parasites carrying Leishmania RNA virus 1 (LRV1) are predisposed to cause aggravated symptoms in the mucocutaneous form of leishmaniasis. Here, we present the structure of the LRV1 virion determined using cryo-electron microscopy. | |||
Virion structure of Leishmania RNA virus 1.,Prochazkova M, Fuzik T, Grybchuk D, Yurchenko V, Plevka P Virology. 2022 Dec;577:149-154. doi: 10.1016/j.virol.2022.09.014. Epub 2022 Nov , 1. PMID:36371873<ref>PMID:36371873</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ns2" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Latest revision as of 14:15, 23 October 2024
Virion of Leishmania RNA virus 1Virion of Leishmania RNA virus 1
Structural highlights
FunctionPublication Abstract from PubMedThe presence of Leishmania RNA virus 1 (LRV1) enables Leishmania protozoan parasites to cause more severe disease than the virus-free strains. The structure of LRV1 virus-like particles has been determined previously, however, the structure of the LRV1 virion has not been characterized. Here we used cryo-electron microscopy and single-particle reconstruction to determine the structures of the LRV1 virion and empty particle isolated from Leishmania guyanensis to resolutions of 4.0 A and 3.6 A, respectively. The capsid of LRV1 is built from sixty dimers of capsid proteins organized with icosahedral symmetry. RNA genomes of totiviruses are replicated inside the virions by RNA polymerases expressed as C-terminal extensions of a sub-population of capsid proteins. Most of the virions probably contain one or two copies of the RNA polymerase, however, the location of the polymerase domains in LRV1 capsid could not be identified, indicating that it varies among particles. Importance. Every year over 200 000 people contract leishmaniasis and more than five hundred people die of the disease. The mucocutaneous form of leishmaniasis produces lesions that can destroy the mucous membranes of the nose, mouth, and throat. Leishmania parasites carrying Leishmania RNA virus 1 (LRV1) are predisposed to cause aggravated symptoms in the mucocutaneous form of leishmaniasis. Here, we present the structure of the LRV1 virion determined using cryo-electron microscopy. Virion structure of Leishmania RNA virus 1.,Prochazkova M, Fuzik T, Grybchuk D, Yurchenko V, Plevka P Virology. 2022 Dec;577:149-154. doi: 10.1016/j.virol.2022.09.014. Epub 2022 Nov , 1. PMID:36371873[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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