7nod: Difference between revisions

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<StructureSection load='7nod' size='340' side='right'caption='[[7nod]], [[Resolution|resolution]] 7.80&Aring;' scene=''>
<StructureSection load='7nod' size='340' side='right'caption='[[7nod]], [[Resolution|resolution]] 7.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7nod]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Rsv-prc Rsv-prc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NOD FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NOD FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11888 RSV-PrC])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nod OCA], [https://pdbe.org/7nod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nod RCSB], [https://www.ebi.ac.uk/pdbsum/7nod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nod ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nod OCA], [https://pdbe.org/7nod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nod RCSB], [https://www.ebi.ac.uk/pdbsum/7nod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nod ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/GAG_RSVP GAG_RSVP]] Capsid protein p27 forms the spherical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity).  The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Inositol hexakisphosphate (IP6) is an assembly cofactor for HIV-1. We report here that IP6 is also used for assembly of Rous sarcoma virus (RSV), a retrovirus from a different genus. IP6 is ~100-fold more potent at promoting RSV mature capsid protein (CA) assembly than observed for HIV-1 and removal of IP6 in cells reduces infectivity by 100-fold. Here, visualized by cryo-electron tomography and subtomogram averaging, mature capsid-like particles show an IP6-like density in the CA hexamer, coordinated by rings of six lysines and six arginines. Phosphate and IP6 have opposing effects on CA in vitro assembly, inducing formation of T = 1 icosahedrons and tubes, respectively, implying that phosphate promotes pentamer and IP6 hexamer formation. Subtomogram averaging and classification optimized for analysis of pleomorphic retrovirus particles reveal that the heterogeneity of mature RSV CA polyhedrons results from an unexpected, intrinsic CA hexamer flexibility. In contrast, the CA pentamer forms rigid units organizing the local architecture. These different features of hexamers and pentamers determine the structural mechanism to form CA polyhedrons of variable shape in mature RSV particles.


Structure of the mature Rous sarcoma virus lattice reveals a role for IP6 in the formation of the capsid hexamer.,Obr M, Ricana CL, Nikulin N, Feathers JR, Klanschnig M, Thader A, Johnson MC, Vogt VM, Schur FKM, Dick RA Nat Commun. 2021 May 28;12(1):3226. doi: 10.1038/s41467-021-23506-0. PMID:34050170<ref>PMID:34050170</ref>
==See Also==
 
*[[Gag polyprotein 3D structures|Gag polyprotein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7nod" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Rsv-prc]]
[[Category: Dick RA]]
[[Category: Dick, R A]]
[[Category: Feathers J-PR]]
[[Category: Feathers, J P.R]]
[[Category: Johnson MC]]
[[Category: Johnson, M C]]
[[Category: Klanschnig M]]
[[Category: Klanschnig, M]]
[[Category: Nikulin N]]
[[Category: Nikulin, N]]
[[Category: Obr M]]
[[Category: Obr, M]]
[[Category: Ricana CL]]
[[Category: Ricana, C L]]
[[Category: Schur FKM]]
[[Category: Schur, F K.M]]
[[Category: Thader A]]
[[Category: Thader, A]]
[[Category: Vogt VM]]
[[Category: Vogt, V M]]
[[Category: Capsid protein]]
[[Category: Ip6]]
[[Category: Retrovirus]]
[[Category: Rous sarcoma virus]]
[[Category: Viral protein]]

Latest revision as of 11:57, 14 July 2024

Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 3'4Structure of the mature RSV CA lattice: Group III, hexamer-hexamer interface, class 3'4

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 7.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

7nod, resolution 7.80Å

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