7b8p: Difference between revisions

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<StructureSection load='7b8p' size='340' side='right'caption='[[7b8p]], [[Resolution|resolution]] 3.54&Aring;' scene=''>
<StructureSection load='7b8p' size='340' side='right'caption='[[7b8p]], [[Resolution|resolution]] 3.54&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7b8p]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B8P FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B8P FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b8p OCA], [https://pdbe.org/7b8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b8p RCSB], [https://www.ebi.ac.uk/pdbsum/7b8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b8p ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.54&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b8p OCA], [https://pdbe.org/7b8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b8p RCSB], [https://www.ebi.ac.uk/pdbsum/7b8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b8p ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Upon antibiotic stress Gram-negative pathogens deploy resistance-nodulation-cell division-type tripartite efflux pumps. These include a H(+)/drug antiporter module that recognizes structurally diverse substances, including antibiotics. Here, we show the 3.5 A structure of subunit AdeB from the Acinetobacter baumannii AdeABC efflux pump solved by single-particle cryo-electron microscopy. The AdeB trimer adopts mainly a resting state with all protomers in a conformation devoid of transport channels or antibiotic binding sites. However, 10% of the protomers adopt a state where three transport channels lead to the closed substrate (deep) binding pocket. A comparison between drug binding of AdeB and Escherichia coli AcrB is made via activity analysis of 20 AdeB variants, selected on basis of side chain interactions with antibiotics observed in the AcrB periplasmic domain X-ray co-structures with fusidic acid (2.3 A), doxycycline (2.1 A) and levofloxacin (2.7 A). AdeABC, compared to AcrAB-TolC, confers higher resistance to E. coli towards polyaromatic compounds and lower resistance towards antibiotic compounds.
Structural and functional analysis of the promiscuous AcrB and AdeB efflux pumps suggests different drug binding mechanisms.,Ornik-Cha A, Wilhelm J, Kobylka J, Sjuts H, Vargiu AV, Malloci G, Reitz J, Seybert A, Frangakis AS, Pos KM Nat Commun. 2021 Nov 25;12(1):6919. doi: 10.1038/s41467-021-27146-2. PMID:34824229<ref>PMID:34824229</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7b8p" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Frangakis, A]]
[[Category: Frangakis A]]
[[Category: Ornik-Cha, A]]
[[Category: Ornik-Cha A]]
[[Category: Pos, K M]]
[[Category: Pos KM]]
[[Category: Reitz, J]]
[[Category: Reitz J]]
[[Category: Seybert, A]]
[[Category: Seybert A]]
[[Category: Antibiotic resistance]]
[[Category: Membrane protein]]
[[Category: Multidrug transporter]]
[[Category: Rnd-transporter]]
[[Category: Transport protein]]

Latest revision as of 11:54, 14 July 2024

Acinetobacter baumannii multidrug transporter AdeB in OOO stateAcinetobacter baumannii multidrug transporter AdeB in OOO state

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.54Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

7b8p, resolution 3.54Å

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
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