7akw: Difference between revisions
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<StructureSection load='7akw' size='340' side='right'caption='[[7akw]], [[Resolution|resolution]] 1.96Å' scene=''> | <StructureSection load='7akw' size='340' side='right'caption='[[7akw]], [[Resolution|resolution]] 1.96Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AKW FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7akw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7akw OCA], [https://pdbe.org/7akw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7akw RCSB], [https://www.ebi.ac.uk/pdbsum/7akw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7akw ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7akw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7akw OCA], [https://pdbe.org/7akw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7akw RCSB], [https://www.ebi.ac.uk/pdbsum/7akw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7akw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Alekseev A]] | [[Category: Alekseev A]] | ||
[[Category: Astashkin R]] | [[Category: Astashkin R]] | ||
Latest revision as of 16:25, 6 November 2024
Crystal structure of the viral rhodopsins chimera O1O2Crystal structure of the viral rhodopsins chimera O1O2
Structural highlights
Publication Abstract from PubMedPhytoplankton is the base of the marine food chain as well as oxygen and carbon cycles and thus plays a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms and regulate the phytoplankton dynamics encompass genes of rhodopsins of two distinct families. Here, we present a functional and structural characterization of two proteins of viral rhodopsin group 1, OLPVR1 and VirChR1. Functional analysis of VirChR1 shows that it is a highly selective, Na(+)/K(+)-conducting channel and, in contrast to known cation channelrhodopsins, it is impermeable to Ca(2+) ions. We show that, upon illumination, VirChR1 is able to drive neural firing. The 1.4 A resolution structure of OLPVR1 reveals remarkable differences from the known channelrhodopsins and a unique ion-conducting pathway. Thus, viral rhodopsins 1 represent a unique, large group of light-gated channels (viral channelrhodopsins, VirChR1s). In nature, VirChR1s likely mediate phototaxis of algae enhancing the host anabolic processes to support virus reproduction, and therefore, might play a major role in global phytoplankton dynamics. Moreover, VirChR1s have unique potential for optogenetics as they lack possibly noxious Ca(2+) permeability. Viral rhodopsins 1 are an unique family of light-gated cation channels.,Zabelskii D, Alekseev A, Kovalev K, Rankovic V, Balandin T, Soloviov D, Bratanov D, Savelyeva E, Podolyak E, Volkov D, Vaganova S, Astashkin R, Chizhov I, Yutin N, Rulev M, Popov A, Eria-Oliveira AS, Rokitskaya T, Mager T, Antonenko Y, Rosselli R, Armeev G, Shaitan K, Vivaudou M, Buldt G, Rogachev A, Rodriguez-Valera F, Kirpichnikov M, Moser T, Offenhausser A, Willbold D, Koonin E, Bamberg E, Gordeliy V Nat Commun. 2020 Nov 11;11(1):5707. doi: 10.1038/s41467-020-19457-7. PMID:33177509[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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