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==== | ==Cryo-EM structure of Spondweni virus prME== | ||
<StructureSection load='6zqi' size='340' side='right'caption='[[6zqi]]' scene=''> | <StructureSection load='6zqi' size='340' side='right'caption='[[6zqi]], [[Resolution|resolution]] 3.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | <table><tr><td colspan='2'>[[6zqi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Spondweni_virus Spondweni virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZQI FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zqi OCA], [https://pdbe.org/6zqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zqi RCSB], [https://www.ebi.ac.uk/pdbsum/6zqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zqi ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zqi OCA], [https://pdbe.org/6zqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zqi RCSB], [https://www.ebi.ac.uk/pdbsum/6zqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zqi ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/C8XPB6_9FLAV C8XPB6_9FLAV] Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Flaviviruses such as Dengue (DENV) or Zika virus (ZIKV) assemble into an immature form within the endoplasmatic reticulum (ER), and are then processed by furin protease in the trans-Golgi. To better grasp maturation, we carry out cryo-EM reconstructions of immature Spondweni virus (SPOV), a human flavivirus of the same serogroup as ZIKV. By employing asymmetric localised reconstruction we push the resolution to 3.8 A, enabling us to refine an atomic model which includes the crucial furin protease recognition site and a conserved Histidine pH-sensor. For direct comparison, we also solve structures of the mature forms of SPONV and DENV to 2.6 A and 3.1 A, respectively. We identify an ordered lipid that is present in only the mature forms of ZIKV, SPOV, and DENV and can bind as a consequence of rearranging amphipathic stem-helices of E during maturation. We propose a structural role for the pocket and suggest it stabilizes mature E. | |||
Flavivirus maturation leads to the formation of an occupied lipid pocket in the surface glycoproteins.,Renner M, Dejnirattisai W, Carrique L, Martin IS, Karia D, Ilca SL, Ho SF, Kotecha A, Keown JR, Mongkolsapaya J, Screaton GR, Grimes JM Nat Commun. 2021 Feb 23;12(1):1238. doi: 10.1038/s41467-021-21505-9. PMID:33623019<ref>PMID:33623019</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6zqi" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Spondweni virus]] | ||
[[Category: Carrique L]] | |||
[[Category: Dejnirattisai W]] | |||
[[Category: Grimes JM]] | |||
[[Category: Ho SF]] | |||
[[Category: Ilca SL]] | |||
[[Category: Karia D]] | |||
[[Category: Keown JR]] | |||
[[Category: Kotecha A]] | |||
[[Category: Mongkolsapaya J]] | |||
[[Category: Renner M]] | |||
[[Category: Screaton GR]] | |||
[[Category: Serna Martin I]] | |||
Latest revision as of 16:22, 6 November 2024
Cryo-EM structure of Spondweni virus prMECryo-EM structure of Spondweni virus prME
Structural highlights
FunctionC8XPB6_9FLAV Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504] Publication Abstract from PubMedFlaviviruses such as Dengue (DENV) or Zika virus (ZIKV) assemble into an immature form within the endoplasmatic reticulum (ER), and are then processed by furin protease in the trans-Golgi. To better grasp maturation, we carry out cryo-EM reconstructions of immature Spondweni virus (SPOV), a human flavivirus of the same serogroup as ZIKV. By employing asymmetric localised reconstruction we push the resolution to 3.8 A, enabling us to refine an atomic model which includes the crucial furin protease recognition site and a conserved Histidine pH-sensor. For direct comparison, we also solve structures of the mature forms of SPONV and DENV to 2.6 A and 3.1 A, respectively. We identify an ordered lipid that is present in only the mature forms of ZIKV, SPOV, and DENV and can bind as a consequence of rearranging amphipathic stem-helices of E during maturation. We propose a structural role for the pocket and suggest it stabilizes mature E. Flavivirus maturation leads to the formation of an occupied lipid pocket in the surface glycoproteins.,Renner M, Dejnirattisai W, Carrique L, Martin IS, Karia D, Ilca SL, Ho SF, Kotecha A, Keown JR, Mongkolsapaya J, Screaton GR, Grimes JM Nat Commun. 2021 Feb 23;12(1):1238. doi: 10.1038/s41467-021-21505-9. PMID:33623019[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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