|
|
| Line 3: |
Line 3: |
| <StructureSection load='6zgd' size='340' side='right'caption='[[6zgd]], [[Resolution|resolution]] 4.10Å' scene=''> | | <StructureSection load='6zgd' size='340' side='right'caption='[[6zgd]], [[Resolution|resolution]] 4.10Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6zgd]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Glovi Glovi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZGD FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZGD FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">glvI, glr4197 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=251221 GLOVI])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zgd OCA], [https://pdbe.org/6zgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zgd RCSB], [https://www.ebi.ac.uk/pdbsum/6zgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zgd ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zgd OCA], [https://pdbe.org/6zgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zgd RCSB], [https://www.ebi.ac.uk/pdbsum/6zgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zgd ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function ==
| |
| [[https://www.uniprot.org/uniprot/GLIC_GLOVI GLIC_GLOVI]] Cationic channel with similar permeabilities for Na(+) and K(+), that is activated by an increase of the proton concentration on the extracellular side. Displays no permeability for chloride ions. Shows slow kinetics of activation, no desensitization and a single channel conductance of 8 pS. Might contribute to adaptation to external pH change.<ref>PMID:17167423</ref>
| |
| <div style="background-color:#fffaf0;">
| |
| == Publication Abstract from PubMed ==
| |
| Ligand-gated ion channels are critical mediators of electrochemical signal transduction across evolution. Biophysical and pharmacological characterization of these receptor proteins relies on high-quality structures in multiple, subtly distinct functional states. However, structural data in this family remain limited, particularly for resting and intermediate states on the activation pathway. Here, we report cryo-electron microscopy (cryo-EM) structures of the proton-activated Gloeobacter violaceus ligand-gated ion channel (GLIC) under three pH conditions. Decreased pH was associated with improved resolution and side chain rearrangements at the subunit/domain interface, particularly involving functionally important residues in the beta1-beta2 and M2-M3 loops. Molecular dynamics simulations substantiated flexibility in the closed-channel extracellular domains relative to the transmembrane ones and supported electrostatic remodeling around E35 and E243 in proton-induced gating. Exploration of secondary cryo-EM classes further indicated a low-pH population with an expanded pore. These results allow us to define distinct protonation and activation steps in pH-stimulated conformational cycling in GLIC, including interfacial rearrangements largely conserved in the pentameric channel family.
| |
|
| |
|
| Dynamic closed states of a ligand-gated ion channel captured by cryo-EM and simulations.,Rovsnik U, Zhuang Y, Forsberg BO, Carroni M, Yvonnesdotter L, Howard RJ, Lindahl E Life Sci Alliance. 2021 Jul 1;4(8). pii: 4/8/e202101011. doi:, 10.26508/lsa.202101011. Print 2021 Aug. PMID:34210687<ref>PMID:34210687</ref>
| | ==See Also== |
| | | *[[Ion channels 3D structures|Ion channels 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 6zgd" style="background-color:#fffaf0;"></div>
| |
| == References == | |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Glovi]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Carroni, M]] | | [[Category: Carroni M]] |
| [[Category: Forsberg, B O]] | | [[Category: Forsberg BO]] |
| [[Category: Howard, R J]] | | [[Category: Howard RJ]] |
| [[Category: Lindahl, E]] | | [[Category: Lindahl E]] |
| [[Category: Rovsnik, U]] | | [[Category: Rovsnik U]] |
| [[Category: Yvonnesdotter, L]] | | [[Category: Yvonnesdotter L]] |
| [[Category: Zhuang, Y]] | | [[Category: Zhuang Y]] |
| [[Category: Cryoem]]
| |
| [[Category: Glic]]
| |
| [[Category: Membrane protein]]
| |
| [[Category: Pentameric ligand-gated ion channel]]
| |