Proteopedia

6w0c: Difference between revisions

← Older edit
No edit summary
No edit summary
 
Line 3: Line 3:
<StructureSection load='6w0c' size='340' side='right'caption='[[6w0c]], [[Resolution|resolution]] 3.56&Aring;' scene=''>
<StructureSection load='6w0c' size='340' side='right'caption='[[6w0c]], [[Resolution|resolution]] 3.56&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6w0c]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] and [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W0C FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W0C FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.556&#8491;</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.556&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w0c OCA], [https://pdbe.org/6w0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w0c RCSB], [https://www.ebi.ac.uk/pdbsum/6w0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w0c ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w0c OCA], [https://pdbe.org/6w0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w0c RCSB], [https://www.ebi.ac.uk/pdbsum/6w0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w0c ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Barium (Ba(2+)) is a classic permeant blocker of potassium (K(+)) channels. The "external lock-in effect" in barium block experiments, whereby the binding of external K(+) impedes the forward translocation of the blocker, provides a powerful avenue to investigate the selectivity of the binding sites along the pore of potassium channels. Barium block experiments show that the external lock-in site is highly selective for K(+) over Na(+). Wild-type KcsA was crystallized in low K(+) conditions, and the crystals were soaked in solutions containing various concentrations of barium. Structural analysis reveals open and closed gate conformations of the KcsA channel. Anomalous diffraction experiments show that Ba(2+) primarily binds to the innermost site S4 of the selectivity filter of the open-gate conformation and also the site S2, but no binding is detected with the closed-gate conformation. Alchemical free-energy perturbation calculations indicate that the presence of a Ba(2+) ion in the selectivity filter boosts the specificity of K(+) binding relative to Na(+) in the external sites S0-S2.
Open and Closed Structures of a Barium-Blocked Potassium Channel.,Rohaim A, Gong L, Li J, Rui H, Blachowicz L, Roux B J Mol Biol. 2020 Aug 7;432(17):4783-4798. doi: 10.1016/j.jmb.2020.06.012. Epub, 2020 Jun 29. PMID:32615129<ref>PMID:32615129</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6w0c" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Streptomyces lividans]]
[[Category: Gong L]]
[[Category: Gong L]]
[[Category: Li J]]
[[Category: Li J]]
[[Category: Rohaim A]]
[[Category: Rohaim A]]

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/w/6w0c"