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| <StructureSection load='6l3v' size='340' side='right'caption='[[6l3v]], [[Resolution|resolution]] 2.63Å' scene=''> | | <StructureSection load='6l3v' size='340' side='right'caption='[[6l3v]], [[Resolution|resolution]] 2.63Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6l3v]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L3V FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L3V FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMN:LAURYL+MALTOSE+NEOPENTYL+GLYCOL'>LMN</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.63Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GJC3, GJE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMN:LAURYL+MALTOSE+NEOPENTYL+GLYCOL'>LMN</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3v OCA], [http://pdbe.org/6l3v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3v RCSB], [http://www.ebi.ac.uk/pdbsum/6l3v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3v ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3v OCA], [https://pdbe.org/6l3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l3v RCSB], [https://www.ebi.ac.uk/pdbsum/6l3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3v ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function ==
| |
| [[http://www.uniprot.org/uniprot/CXG3_HUMAN CXG3_HUMAN]] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Connexin family proteins assemble into hexameric channels called hemichannels/connexons, which function as transmembrane channels or dock together to form gap junction intercellular channels (GJIChs). We determined the cryo-electron microscopy structures of human connexin 31.3 (Cx31.3)/GJC3 hemichannels in the presence and absence of calcium ions and with a hearing-loss mutation R15G at 2.3-, 2.5-, and 2.6-A resolutions, respectively. Compared with available structures of GJICh in open conformation, Cx31.3 hemichannel shows substantial structural changes of highly conserved regions in the connexin family, including opening of calcium ion-binding tunnels, reorganization of salt-bridge networks, exposure of lipid-binding sites, and collocation of amino-terminal helices at the cytoplasmic entrance. We also found that the hemichannel has a pore with a diameter of ~8 A and selectively transports chloride ions. Our study provides structural insights into the permeant selectivity of Cx31.3 hemichannel.
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| Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel.,Lee HJ, Jeong H, Hyun J, Ryu B, Park K, Lim HH, Yoo J, Woo JS Sci Adv. 2020 Aug 28;6(35):eaba4996. doi: 10.1126/sciadv.aba4996. eCollection, 2020 Aug. PMID:32923625<ref>PMID:32923625</ref>
| | ==See Also== |
| | | *[[Connexin 3D structure|Connexin 3D structure]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6l3v" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Human]]
| |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Hyun, J]] | | [[Category: Hyun J]] |
| [[Category: Jeong, H]] | | [[Category: Jeong H]] |
| [[Category: Lee, H J]] | | [[Category: Lee HJ]] |
| [[Category: Ryu, B]] | | [[Category: Ryu B]] |
| [[Category: Woo, J S]] | | [[Category: Woo JS]] |
| [[Category: Atp release]]
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| [[Category: Gap junction]]
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| [[Category: Hemichannel]]
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| [[Category: Hexamer]]
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| [[Category: Membrane protein]]
| |